5fn8: Difference between revisions
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<StructureSection load='5fn8' size='340' side='right'caption='[[5fn8]], [[Resolution|resolution]] 2.45Å' scene=''> | <StructureSection load='5fn8' size='340' side='right'caption='[[5fn8]], [[Resolution|resolution]] 2.45Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5fn8]] is a 2 chain structure with sequence from [ | <table><tr><td colspan='2'>[[5fn8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FN8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FN8 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fn8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fn8 OCA], [https://pdbe.org/5fn8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fn8 RCSB], [https://www.ebi.ac.uk/pdbsum/5fn8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fn8 ProSAT]</span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/PTPRC_RAT PTPRC_RAT] Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN (By similarity). Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Rattus norvegicus]] | ||
[[Category: Aricescu | [[Category: Aricescu AR]] | ||
[[Category: Chang | [[Category: Chang VT]] | ||
[[Category: Coles | [[Category: Coles CH]] | ||
[[Category: Davis | [[Category: Davis SJ]] | ||
[[Category: Fernandes | [[Category: Fernandes RA]] | ||
[[Category: Ganzinger | [[Category: Ganzinger KA]] | ||
[[Category: Gilbert | [[Category: Gilbert RJC]] | ||
[[Category: Harlos | [[Category: Harlos K]] | ||
[[Category: Huang | [[Category: Huang E]] | ||
[[Category: Jones | [[Category: Jones EY]] | ||
[[Category: Jonsson | [[Category: Jonsson P]] | ||
[[Category: Klenerman | [[Category: Klenerman D]] | ||
[[Category: Lee | [[Category: Lee SF]] | ||
[[Category: Lui | [[Category: Lui Y]] | ||
[[Category: McColl | [[Category: McColl J]] | ||
[[Category: Palayret | [[Category: Palayret M]] | ||
[[Category: Siebold | [[Category: Siebold C]] | ||
Latest revision as of 09:59, 19 July 2023
Crystal structure of rat CD45 extracellular region, domains d3-d4Crystal structure of rat CD45 extracellular region, domains d3-d4
Structural highlights
FunctionPTPRC_RAT Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN (By similarity). Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). Publication Abstract from PubMedIt has been proposed that the local segregation of kinases and the tyrosine phosphatase CD45 underpins T cell antigen receptor (TCR) triggering, but how such segregation occurs and whether it can initiate signaling is unclear. Using structural and biophysical analysis, we show that the extracellular region of CD45 is rigid and extends beyond the distance spanned by TCR-ligand complexes, implying that sites of TCR-ligand engagement would sterically exclude CD45. We also show that the formation of 'close contacts', new structures characterized by spontaneous CD45 and kinase segregation at the submicron-scale, initiates signaling even when TCR ligands are absent. Our work reveals the structural basis for, and the potent signaling effects of, local CD45 and kinase segregation. TCR ligands have the potential to heighten signaling simply by holding receptors in close contacts. Initiation of T cell signaling by CD45 segregation at 'close contacts'.,Chang VT, Fernandes RA, Ganzinger KA, Lee SF, Siebold C, McColl J, Jonsson P, Palayret M, Harlos K, Coles CH, Jones EY, Lui Y, Huang E, Gilbert RJ, Klenerman D, Aricescu AR, Davis SJ Nat Immunol. 2016 May;17(5):574-82. doi: 10.1038/ni.3392. Epub 2016 Mar 21. PMID:26998761[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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