8j62: Difference between revisions

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-'''Unreleased structure'''
-The entry 8j62 is ON HOLD  until 2025-04-24
+==Cryo-EM structure of APOBEC3G-Vif complex==
+<StructureSection load='8j62' size='340' side='right'caption='[[8j62]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[8j62]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens], [https://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8J62 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8J62 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8j62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8j62 OCA], [https://pdbe.org/8j62 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8j62 RCSB], [https://www.ebi.ac.uk/pdbsum/8j62 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8j62 ProSAT]</span></td></tr>
+</table>
+== Disease ==
+[https://www.uniprot.org/uniprot/PEBB_HUMAN PEBB_HUMAN] Note=A chromosomal aberration involving CBFB is associated with acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein that consists of the 165 N-terminal residues of CBF-beta (PEPB2) with the tail region of MYH11.
+== Function ==
+[https://www.uniprot.org/uniprot/PEBB_HUMAN PEBB_HUMAN] CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. CBFB enhances DNA binding by RUNX1.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Great effort has been devoted to discovering the basis of A3G-Vif interaction, the key event of HIV's counteraction mechanism to evade antiviral innate immune response. Here we show reconstitution of the A3G-Vif complex and subsequent A3G ubiquitination in vitro and report the cryo-EM structure of the A3G-Vif complex at 2.8 A resolution using solubility-enhanced variants of A3G and Vif. We present an atomic model of the A3G-Vif interface, which assembles via known amino acid determinants. This assembly is not achieved by protein-protein interaction alone, but also involves RNA. The cryo-EM structure and in vitro ubiquitination assays identify an adenine/guanine base preference for the interaction and a unique Vif-ribose contact. This establishes the biological significance of an RNA ligand. Further assessment of interactions between A3G, Vif, and RNA ligands show that the A3G-Vif assembly and subsequent ubiquitination can be controlled by amino acid mutations at the interface or by polynucleotide modification, suggesting that a specific chemical moiety would be a promising pharmacophore to inhibit the A3G-Vif interaction.
-Authors:
+Structural insights into RNA bridging between HIV-1 Vif and antiviral factor APOBEC3G.,Kouno T, Shibata S, Shigematsu M, Hyun J, Kim TG, Matsuo H, Wolf M Nat Commun. 2023 Jul 7;14(1):4037. doi: 10.1038/s41467-023-39796-5. PMID:37419875<ref>PMID:37419875</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 8j62" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Homo sapiens]]
+[[Category: Human immunodeficiency virus 1]]
+[[Category: Large Structures]]
+[[Category: Synthetic construct]]
+[[Category: Hyun J]]
+[[Category: Kim TG]]
+[[Category: Kouno T]]
+[[Category: Shibata S]]
+[[Category: Wolf M]]