1ll5: Difference between revisions

Revision as of 00:01, 3 May 2008

X-ray crystal structure of AmpC WT beta-lactamase in complex with covalently bound imipenem

OverviewOverview

To determine how imipenem inhibits the class C beta-lactamase AmpC, the X-ray crystal structure of the acyl-enzyme complex was determined to a resolution of 1.80 A. In the complex, the lactam carbonyl oxygen of imipenem has flipped by approximately 180 degrees compared to its expected position; the electrophilic acyl center is thus displaced from the point of hydrolytic attack. This conformation resembles that of imipenem bound to the class A enzyme TEM-1 but is different from that of moxalactam bound to AmpC.

About this StructureAbout this Structure

1LL5 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis for imipenem inhibition of class C beta-lactamases., Beadle BM, Shoichet BK, Antimicrob Agents Chemother. 2002 Dec;46(12):3978-80. PMID:12435704 Page seeded by OCA on Sat May 3 00:01:42 2008

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OCA

@@ Line 1: / Line 1: @@
 [[Image:1ll5.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1ll5 |SIZE=350|CAPTION= <scene name='initialview01'>1ll5</scene>, resolution 1.80&Aring;
+The line below this paragraph, containing "STRUCTURE_1ll5", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=IM2:3-(2-FORMIMIDOYLAMINO-ETHYLSULFANYL)-5-(1-FORMYL-2-HYDROXY-PROPYL)-4,5-DIHYDRO-1H-PYRROLE-2-CARBOXYLIC+ACID'>IM2</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= K12 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+-->
-|DOMAIN=
+{{STRUCTURE_1ll5|  PDB=1ll5  |  SCENE=  }}
-|RELATEDENTRY=[[1ke4|1KE4]], [[1fco|1FCO]], [[1bt5|1BT5]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ll5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ll5 OCA], [http://www.ebi.ac.uk/pdbsum/1ll5 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ll5 RCSB]</span>
-}}
 '''X-ray crystal structure of AmpC WT beta-lactamase in complex with covalently bound imipenem'''
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 [[Category: Beadle, B M.]]
 [[Category: Shoichet, B K.]]
-[[Category: beta-lactamase]]
+[[Category: Beta-lactamase]]
-[[Category: carbapenem]]
+[[Category: Carbapenem]]
-[[Category: imipenem]]
+[[Category: Imipenem]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 00:01:42 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:04:09 2008''