8fnc: Difference between revisions
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==Cryo-EM structure of RNase-treated RESC-C in trypanosomal RNA editing== | |||
<StructureSection load='8fnc' size='340' side='right'caption='[[8fnc]], [[Resolution|resolution]] 3.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8fnc]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_brucei Trypanosoma brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8FNC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8FNC FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.3Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8fnc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8fnc OCA], [https://pdbe.org/8fnc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8fnc RCSB], [https://www.ebi.ac.uk/pdbsum/8fnc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8fnc ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q389W4_TRYB2 Q389W4_TRYB2] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
In Trypanosoma brucei, the editosome, composed of RNA-editing substrate-binding complex (RESC) and RNA-editing catalytic complex (RECC), orchestrates guide RNA (gRNA)-programmed editing to recode cryptic mitochondrial transcripts into messenger RNAs (mRNAs). The mechanism of information transfer from gRNA to mRNA is unclear owing to a lack of high-resolution structures for these complexes. With cryo-electron microscopy and functional studies, we have captured gRNA-stabilizing RESC-A and gRNA-mRNA-binding RESC-B and RESC-C particles. RESC-A sequesters gRNA termini, thus promoting hairpin formation and blocking mRNA access. The conversion of RESC-A into RESC-B or -C unfolds gRNA and allows mRNA selection. The ensuing gRNA-mRNA duplex protrudes from RESC-B, likely exposing editing sites to RECC-catalyzed cleavage, uridine insertion or deletion, and ligation. Our work reveals a remodeling event facilitating gRNA-mRNA hybridization and assembly of a macromolecular substrate for the editosome's catalytic modality. | |||
Structural basis of gRNA stabilization and mRNA recognition in trypanosomal RNA editing.,Liu S, Wang H, Li X, Zhang F, Lee JKJ, Li Z, Yu C, Hu JJ, Zhao X, Suematsu T, Alvarez-Cabrera AL, Liu Q, Zhang L, Huang L, Aphasizheva I, Aphasizhev R, Zhou ZH Science. 2023 Jul 7;381(6653):eadg4725. doi: 10.1126/science.adg4725. Epub 2023 , Jul 7. PMID:37410820<ref>PMID:37410820</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 8fnc" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Trypanosoma brucei]] | |||
[[Category: Alvarez-Cabrera AL]] | |||
[[Category: Aphasizhev R]] | |||
[[Category: Aphasizheva I]] | |||
[[Category: Hu JJ]] | |||
[[Category: Huang L]] | |||
[[Category: Lee JKJ]] | |||
[[Category: Li X]] | |||
[[Category: Li Z]] | |||
[[Category: Liu Q]] | |||
[[Category: Liu S]] | |||
[[Category: Suematsu T]] | |||
[[Category: Wang H]] | |||
[[Category: Yu C]] | |||
[[Category: Zhang F]] | |||
[[Category: Zhang L]] | |||
[[Category: Zhao X]] | |||
[[Category: Zhou ZH]] | |||
Revision as of 09:13, 19 July 2023
Cryo-EM structure of RNase-treated RESC-C in trypanosomal RNA editingCryo-EM structure of RNase-treated RESC-C in trypanosomal RNA editing
Structural highlights
FunctionPublication Abstract from PubMedIn Trypanosoma brucei, the editosome, composed of RNA-editing substrate-binding complex (RESC) and RNA-editing catalytic complex (RECC), orchestrates guide RNA (gRNA)-programmed editing to recode cryptic mitochondrial transcripts into messenger RNAs (mRNAs). The mechanism of information transfer from gRNA to mRNA is unclear owing to a lack of high-resolution structures for these complexes. With cryo-electron microscopy and functional studies, we have captured gRNA-stabilizing RESC-A and gRNA-mRNA-binding RESC-B and RESC-C particles. RESC-A sequesters gRNA termini, thus promoting hairpin formation and blocking mRNA access. The conversion of RESC-A into RESC-B or -C unfolds gRNA and allows mRNA selection. The ensuing gRNA-mRNA duplex protrudes from RESC-B, likely exposing editing sites to RECC-catalyzed cleavage, uridine insertion or deletion, and ligation. Our work reveals a remodeling event facilitating gRNA-mRNA hybridization and assembly of a macromolecular substrate for the editosome's catalytic modality. Structural basis of gRNA stabilization and mRNA recognition in trypanosomal RNA editing.,Liu S, Wang H, Li X, Zhang F, Lee JKJ, Li Z, Yu C, Hu JJ, Zhao X, Suematsu T, Alvarez-Cabrera AL, Liu Q, Zhang L, Huang L, Aphasizheva I, Aphasizhev R, Zhou ZH Science. 2023 Jul 7;381(6653):eadg4725. doi: 10.1126/science.adg4725. Epub 2023 , Jul 7. PMID:37410820[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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