1lk2: Difference between revisions

Revision as of 23:59, 2 May 2008

1.35A crystal structure of H-2Kb complexed with the GNYSFYAL peptide

OverviewOverview

We identify and consider some characteristics of a peptide antagonist for the Ag-specific receptor on 2C cells (the 2C TCR). The peptide, GNYSFYAL (called GNY), binds to H-2K(b), and a very high-resolution crystal structure of the GNY-K(b) complex at 1.35 A is described. Although the GNY peptide does not bind to L(d), the potency of GNY-K(b) as an antagonist is evident from its ability to specifically inhibit 2C TCR-mediated reactions to an allogenic agonist complex (QLSPFPFDL-L(d)), as well as to a syngeneic agonist complex (SIYRYYGL-K(b)). The crystal structure and the activities of alanine-substituted peptide variants point to the properties of the peptide P4 side chain and the conformation of the Tyr-P6 side chain as the structural determinants of GNYSFYAL antagonist activity.

About this StructureAbout this Structure

1LK2 is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.

ReferenceReference

A peptide that antagonizes TCR-mediated reactions with both syngeneic and allogeneic agonists: functional and structural aspects., Rudolph MG, Shen LQ, Lamontagne SA, Luz JG, Delaney JR, Ge Q, Cho BK, Palliser D, McKinley CA, Chen J, Wilson IA, Eisen HN, J Immunol. 2004 Mar 1;172(5):2994-3002. PMID:14978103 Page seeded by OCA on Fri May 2 23:59:38 2008

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OCA

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 [[Image:1lk2.gif|left|200px]]
- {{Structure
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lk2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lk2 OCA], [http://www.ebi.ac.uk/pdbsum/1lk2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1lk2 RCSB]</span>
-}}
 '''1.35A crystal structure of H-2Kb complexed with the GNYSFYAL peptide'''
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 [[Category: Rudolph, M G.]]
 [[Category: Wilson, I A.]]
-[[Category: anisotropic and tls refinement]]
+[[Category: Anisotropic and tls refinement]]
-[[Category: class i mhc-peptide complex]]
+[[Category: Class i mhc-peptide complex]]
-[[Category: high resolution]]
+[[Category: High resolution]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 23:59:38 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:03:48 2008''