1ljm: Difference between revisions

Revision as of 23:58, 2 May 2008

DNA recognition is mediated by conformational transition and by DNA bending

OverviewOverview

The Runt domain proteins are transcription regulators of major developmental pathways. Here we present the crystal structures of the Runt domain (RD) of the human protein RUNX1 and its DNA binding site in their free states and compare them with the published crystal structures of RD bound to DNA and to the partner protein CBFbeta. We demonstrate that (1) RD undergoes an allosteric transition upon DNA binding, which is further stabilized by CBFbeta, and that (2) the free DNA target adopts a bent-helical conformation compatible with that of the complex. These findings elucidate the mechanism by which CBFbeta enhances RD binding to DNA as well as the role of the intrinsic conformation of the DNA target in the recognition process.

About this StructureAbout this Structure

1LJM is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

DNA recognition by the RUNX1 transcription factor is mediated by an allosteric transition in the RUNT domain and by DNA bending., Bartfeld D, Shimon L, Couture GC, Rabinovich D, Frolow F, Levanon D, Groner Y, Shakked Z, Structure. 2002 Oct;10(10):1395-407. PMID:12377125 Page seeded by OCA on Fri May 2 23:58:47 2008

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OCA

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 [[Image:1ljm.gif|left|200px]]
- {{Structure
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-|GENE= human ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ljm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ljm OCA], [http://www.ebi.ac.uk/pdbsum/1ljm PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ljm RCSB]</span>
-}}
 '''DNA recognition is mediated by conformational transition and by DNA bending'''
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 [[Category: Shakked, Z.]]
 [[Category: Shimon, L.]]
-[[Category: beta-sandwich]]
+[[Category: Beta-sandwich]]
-[[Category: immunoglobulin fold]]
+[[Category: Immunoglobulin fold]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 23:58:47 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:03:32 2008''