5eqw: Difference between revisions
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<StructureSection load='5eqw' size='340' side='right'caption='[[5eqw]], [[Resolution|resolution]] 1.68Å' scene=''> | <StructureSection load='5eqw' size='340' side='right'caption='[[5eqw]], [[Resolution|resolution]] 1.68Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5eqw]] is a 5 chain structure with sequence from [ | <table><tr><td colspan='2'>[[5eqw]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Acidianus_tailed_spindle_virus Acidianus tailed spindle virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EQW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EQW FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.679Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5eqw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eqw OCA], [https://pdbe.org/5eqw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5eqw RCSB], [https://www.ebi.ac.uk/pdbsum/5eqw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5eqw ProSAT]</span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A125SJ78_9VIRU A0A125SJ78_9VIRU] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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[[Category: Acidianus tailed spindle virus]] | [[Category: Acidianus tailed spindle virus]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Hochstein | [[Category: Hochstein RA]] | ||
[[Category: Lawrence | [[Category: Lawrence CM]] | ||
[[Category: Lintner | [[Category: Lintner NG]] | ||
[[Category: Young | [[Category: Young MJ]] | ||
Latest revision as of 11:17, 12 July 2023
Structure of the major structural protein D135 of Acidianus tailed spindle virus (ATSV)Structure of the major structural protein D135 of Acidianus tailed spindle virus (ATSV)
Structural highlights
FunctionPublication Abstract from PubMedThe spindle-shaped virion morphology is common among archaeal viruses, where it is a defining characteristic of many viral families. However, structural heterogeneity intrinsic to spindle-shaped viruses has seriously hindered efforts to elucidate the molecular architecture of these lemon-shaped capsids. We have utilized a combination of cryo-electron microscopy and X-ray crystallography to study Acidianus tailed spindle virus (ATSV). These studies reveal the architectural principles that underlie assembly of a spindle-shaped virus. Cryo-electron tomography shows a smooth transition from the spindle-shaped capsid into the tubular-shaped tail and allows low-resolution structural modeling of individual virions. Remarkably, higher-dose 2D micrographs reveal a helical surface lattice in the spindle-shaped capsid. Consistent with this, crystallographic studies of the major capsid protein reveal a decorated four-helix bundle that packs within the crystal to form a four-start helical assembly with structural similarity to the tube-shaped tail structure of ATSV and other tailed, spindle-shaped viruses. Combined, this suggests that the spindle-shaped morphology of the ATSV capsid is formed by a multistart helical assembly with a smoothly varying radius and allows construction of a pseudoatomic model for the lemon-shaped capsid that extends into a tubular tail. The potential advantages that this novel architecture conveys to the life cycle of spindle-shaped viruses, including a role in DNA ejection, are discussed. Structural studies of Acidianus tailed spindle virus reveal a structural paradigm used in the assembly of spindle-shaped viruses.,Hochstein R, Bollschweiler D, Dharmavaram S, Lintner NG, Plitzko JM, Bruinsma R, Engelhardt H, Young MJ, Klug WS, Lawrence CM Proc Natl Acad Sci U S A. 2018 Feb 27;115(9):2120-2125. doi:, 10.1073/pnas.1719180115. Epub 2018 Feb 12. PMID:29440399[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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