1lj2: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1lj2.gif|left|200px]] | [[Image:1lj2.gif|left|200px]] | ||
<!-- | |||
The line below this paragraph, containing "STRUCTURE_1lj2", creates the "Structure Box" on the page. | |||
You may change the PDB parameter (which sets the PDB file loaded into the applet) | |||
or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |||
| | or leave the SCENE parameter empty for the default display. | ||
| | --> | ||
{{STRUCTURE_1lj2| PDB=1lj2 | SCENE= }} | |||
}} | |||
'''Recognition of eIF4G by Rotavirus NSP3 reveals a basis for mRNA circularization''' | '''Recognition of eIF4G by Rotavirus NSP3 reveals a basis for mRNA circularization''' | ||
| Line 27: | Line 24: | ||
[[Category: Burley, S K.]] | [[Category: Burley, S K.]] | ||
[[Category: Groft, C M.]] | [[Category: Groft, C M.]] | ||
[[Category: | [[Category: Closed loop]] | ||
[[Category: | [[Category: Coiled coil]] | ||
[[Category: | [[Category: Eif4g]] | ||
[[Category: | [[Category: Homodimer]] | ||
[[Category: | [[Category: Mrna]] | ||
[[Category: | [[Category: Nsp3]] | ||
[[Category: | [[Category: Rotavirus]] | ||
[[Category: | [[Category: Translation]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:58:01 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 23:58, 2 May 2008
Recognition of eIF4G by Rotavirus NSP3 reveals a basis for mRNA circularization
OverviewOverview
Rotaviruses, segmented double-stranded RNA viruses, co-opt the eukaryotic translation machinery with the aid of nonstructural protein 3 (NSP3), a rotaviral functional homolog of the cellular poly(A) binding protein (PABP). NSP3 binds to viral mRNA 3' consensus sequences and circularizes mRNA via interactions with eIF4G. Here, we present the X-ray structure of the C-terminal domain of NSP3 (NSP3-C) recognizing a fragment of eIF4GI. Homodimerization of NSP3-C yields a symmetric, elongated, largely alpha-helical structure with two hydrophobic eIF4G binding pockets at the dimer interface. Site-directed mutagenesis and isothermal titration calorimetry documented that NSP3 and PABP use analogous eIF4G recognition strategies, despite marked differences in tertiary structure.
About this StructureAbout this Structure
1LJ2 is a Protein complex structure of sequences from Simian rotavirus a/sa11. Full crystallographic information is available from OCA.
ReferenceReference
Recognition of eIF4G by rotavirus NSP3 reveals a basis for mRNA circularization., Groft CM, Burley SK, Mol Cell. 2002 Jun;9(6):1273-83. PMID:12086624 Page seeded by OCA on Fri May 2 23:58:01 2008