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==Crystal structure of LOV2-Zdk1 - the complex of oat LOV2 and the affibody protein Zdark1== | ==Crystal structure of LOV2-Zdk1 - the complex of oat LOV2 and the affibody protein Zdark1== | ||
<StructureSection load='5efw' size='340' side='right' caption='[[5efw]], [[Resolution|resolution]] 2.10Å' scene=''> | <StructureSection load='5efw' size='340' side='right'caption='[[5efw]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5efw]] is a 3 chain structure with sequence from [ | <table><tr><td colspan='2'>[[5efw]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Avena_sativa Avena sativa] and [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EFW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EFW FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
< | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5efw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5efw OCA], [https://pdbe.org/5efw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5efw RCSB], [https://www.ebi.ac.uk/pdbsum/5efw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5efw ProSAT]</span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/O49003_AVESA O49003_AVESA] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
| Line 22: | Line 23: | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Avena sativa]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Staphylococcus aureus]] | ||
[[Category: | [[Category: Hahn K]] | ||
[[Category: | [[Category: Hartmann E]] | ||
[[Category: | [[Category: Schlichting I]] | ||
[[Category: | [[Category: Wang H]] | ||
[[Category: | [[Category: Winkler A]] | ||
Latest revision as of 09:24, 5 July 2023
Crystal structure of LOV2-Zdk1 - the complex of oat LOV2 and the affibody protein Zdark1Crystal structure of LOV2-Zdk1 - the complex of oat LOV2 and the affibody protein Zdark1
Structural highlights
FunctionPublication Abstract from PubMedLOVTRAP is an optogenetic approach for reversible light-induced protein dissociation using protein A fragments that bind to the LOV domain only in the dark, with tunable kinetics and a >150-fold change in the dissociation constant (Kd). By reversibly sequestering proteins at mitochondria, we precisely modulated the proteins' access to the cell edge, demonstrating a naturally occurring 3-mHz cell-edge oscillation driven by interactions of Vav2, Rac1, and PI3K proteins. LOVTRAP: an optogenetic system for photoinduced protein dissociation.,Wang H, Vilela M, Winkler A, Tarnawski M, Schlichting I, Yumerefendi H, Kuhlman B, Liu R, Danuser G, Hahn KM Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926. PMID:27427858[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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