5ea2: Difference between revisions
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==Crystal Structure of Holo NAD(P)H dehydrogenase, quinone 1== | ==Crystal Structure of Holo NAD(P)H dehydrogenase, quinone 1== | ||
<StructureSection load='5ea2' size='340' side='right' caption='[[5ea2]], [[Resolution|resolution]] 2.01Å' scene=''> | <StructureSection load='5ea2' size='340' side='right'caption='[[5ea2]], [[Resolution|resolution]] 2.01Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5ea2]] is a 4 chain structure with sequence from [ | <table><tr><td colspan='2'>[[5ea2]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EA2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EA2 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.01Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr> | |||
<tr id=' | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ea2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ea2 OCA], [https://pdbe.org/5ea2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ea2 RCSB], [https://www.ebi.ac.uk/pdbsum/5ea2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ea2 ProSAT]</span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/NQO1_HUMAN NQO1_HUMAN] The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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</div> | </div> | ||
<div class="pdbe-citations 5ea2" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 5ea2" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Quinone reductase|Quinone reductase]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Homo sapiens]] | ||
[[Category: Ahmad | [[Category: Large Structures]] | ||
[[Category: Emadi | [[Category: Ahmad M]] | ||
[[Category: Mbimba | [[Category: Emadi A]] | ||
[[Category: Pidugu | [[Category: Mbimba JE]] | ||
[[Category: Pozharski | [[Category: Pidugu LS]] | ||
[[Category: Sausville | [[Category: Pozharski E]] | ||
[[Category: Toth | [[Category: Sausville EA]] | ||
[[Category: Toth EA]] | |||
Latest revision as of 09:18, 5 July 2023
Crystal Structure of Holo NAD(P)H dehydrogenase, quinone 1Crystal Structure of Holo NAD(P)H dehydrogenase, quinone 1
Structural highlights
FunctionNQO1_HUMAN The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. Publication Abstract from PubMedBACKGROUND: Multimeric naphthoquinones are redox-active compounds that exhibit antineoplastic, antiprotozoal, and antiviral activities. Due to their multimodal effect on perturbation of cellular oxidative state, these compounds hold great potential as therapeutic agents against highly proliferative neoplastic cells. In our previous work, we developed a series of novel dimeric naphthoquinones and showed that they were selectively cytotoxic to human acute myeloid leukemia (AML), breast and prostate cancer cell lines. We subsequently identified the oxidoreductase NAD(P)H dehydrogenase, quinone 1 (NQO1) as the major target of dimeric naphthoquinones and proposed a mechanism of action that entailed induction of a futile redox cycling. RESULTS: Here, for the first time, we describe a direct physical interaction between the bromohydroxy dimeric naphthoquinone E6a and NQO1. Moreover, our studies reveal an extensive binding interface between E6a and the isoalloxazine ring of the flavin adenine dinucleotide (FAD) cofactor of NQO1 in addition to interactions with protein side chains in the active site. We also present biochemical evidence that dimeric naphthoquinones affect the redox state of the FAD cofactor of NQO1. Comparison of the mode of binding of E6a with those of other chemotherapeutics reveals unique characteristics of the interaction that can be leveraged in future drug optimization efforts. CONCLUSION: The first structure of a dimeric naphthoquinone-NQO1 complex was reported, which can be used for design and synthesis of more potent next generation dimeric naphthoquinones to target NQO1 with higher affinity and specificity. A direct interaction between NQO1 and a chemotherapeutic dimeric naphthoquinone.,Pidugu LS, Mbimba JC, Ahmad M, Pozharski E, Sausville EA, Emadi A, Toth EA BMC Struct Biol. 2016 Jan 28;16(1):1. doi: 10.1186/s12900-016-0052-x. PMID:26822308[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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