1ldc: Difference between revisions

Revision as of 23:48, 2 May 2008

X-RAY STRUCTURE OF TWO COMPLEXES OF THE Y143F FLAVOCYTOCHROME B2 MUTANT CRYSTALLIZED IN THE PRESENCE OF LACTATE OR PHENYL-LACTATE

OverviewOverview

Flavocytochrome b2 is a flavohemo enzyme localized in the intermembrane space of yeast mitochondria, where it catalyzes the electron transfer from its substrate, L-lactate, to cytochrome c. We have obtained crystals of a flavocytochrome b2 mutant, Y143F, which are isostructural with those of the native recombinant enzyme [Tegoni, M., & Cambillau, C. (1994) Protein Sci.3, 303-314]. These crystals were grown under similar conditions to those used to obtain the recombinant enzyme, but in the presence of phenyl lactate or lactate. We report here on the structural analysis of the two complexes of flavocytochrome b2 with the reaction products at 2.9 A resolution. In both structures, the Phe143 phenyl ring keeps the same position as that of the phenolic ring of Tyr143 in both the native recombinant and in the native wild-type enzymes. The product of the reaction, phenyl pyruvate or pyruvate, is present at the active site of both subunits, and not only in subunit 2 as observed in the wild-type structure [Xia, Z.-X., & Mathews, F.S. (1990) J. Mol. Biol. 212, 837-863]. The number of interactions between the FMN and the heme domain is considerably lower in the Y143F mutant than in the native proteins. The latter finding strongly supports the hypothesis that the main role of Tyr143 in the native proteins. The latter findings strongly supports the hypothesis that the main role of Tyr143 in the native protein probably consists in establishing a hydrogen bond with the heme [Xia, Z.-X., & Mathews, F.S. (1990) J. Mol. Biol. 212, 837-863]. This interaction appears to be essential for the two domains to approach each other suitably so that the intramolecular electron transfer can occur.

About this StructureAbout this Structure

1LDC is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

ReferenceReference

X-ray structure of two complexes of the Y143F flavocytochrome b2 mutant crystallized in the presence of lactate or phenyl lactate., Tegoni M, Begotti S, Cambillau C, Biochemistry. 1995 Aug 8;34(31):9840-50. PMID:7632684 Page seeded by OCA on Fri May 2 23:48:42 2008

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OCA

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 [[Image:1ldc.jpg|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1ldc", creates the "Structure Box" on the page.
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-|LIGAND= <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=PYR:PYRUVIC+ACID'>PYR</scene>
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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/L-lactate_dehydrogenase_(cytochrome) L-lactate dehydrogenase (cytochrome)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.2.3 1.1.2.3] </span>
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-|GENE= PGR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
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-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ldc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ldc OCA], [http://www.ebi.ac.uk/pdbsum/1ldc PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ldc RCSB]</span>
-}}
 '''X-RAY STRUCTURE OF TWO COMPLEXES OF THE Y143F FLAVOCYTOCHROME B2 MUTANT CRYSTALLIZED IN THE PRESENCE OF LACTATE OR PHENYL-LACTATE'''
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 ==Reference==
 X-ray structure of two complexes of the Y143F flavocytochrome b2 mutant crystallized in the presence of lactate or phenyl lactate., Tegoni M, Begotti S, Cambillau C, Biochemistry. 1995 Aug 8;34(31):9840-50. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7632684 7632684]
-[[Category: L-lactate dehydrogenase (cytochrome)]]
 [[Category: Saccharomyces cerevisiae]]
 [[Category: Single protein]]
 [[Category: Cambillau, C.]]
 [[Category: Tegoni, M.]]
-[[Category: oxidoreductase (flavoenzyme)]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 23:48:42 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:01:25 2008''