1lba: Difference between revisions

Revision as of 23:45, 2 May 2008

THE STRUCTURE OF BACTERIOPHAGE T7 LYSOZYME, A ZINC AMIDASE AND AN INHIBITOR OF T7 RNA POLYMERASE

OverviewOverview

The lysozyme of bacteriophage T7 is a bifunctional protein that cuts amide bonds in the bacterial cell wall and binds to and inhibits transcription by T7 RNA polymerase. The structure of a mutant T7 lysozyme has been determined by x-ray crystallography and refined at 2.2-A resolution. The protein folds into an alpha/beta-sheet structure that has a prominent cleft. A zinc atom is located in the cleft, bound directly to three amino acids and, through a water molecule, to a fourth. Zinc is required for amidase activity but not for inhibition of T7 RNA polymerase. Alignment of the zinc ligands of T7 lysozyme with those of carboxypeptidase A and thermolysin suggests structural similarity among the catalytic sites for the amidase and these zinc proteases. Mutational analysis identified presumed catalytic residues for amidase activity within the cleft and a surface that appears to be the site of binding to T7 RNA polymerase. Binding of T7 RNA polymerase inhibits amidase activity.

About this StructureAbout this Structure

1LBA is a Single protein structure of sequence from Enterobacteria phage t7. Full crystallographic information is available from OCA.

ReferenceReference

The structure of bacteriophage T7 lysozyme, a zinc amidase and an inhibitor of T7 RNA polymerase., Cheng X, Zhang X, Pflugrath JW, Studier FW, Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):4034-8. PMID:8171031 Page seeded by OCA on Fri May 2 23:45:07 2008

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OCA

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 [[Image:1lba.gif|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1lba", creates the "Structure Box" on the page.
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-|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/N-acetylmuramoyl-L-alanine_amidase N-acetylmuramoyl-L-alanine amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.28 3.5.1.28] </span>
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-|GENE= T7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10760 Enterobacteria phage T7])
+-->
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lba FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lba OCA], [http://www.ebi.ac.uk/pdbsum/1lba PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1lba RCSB]</span>
-}}
 '''THE STRUCTURE OF BACTERIOPHAGE T7 LYSOZYME, A ZINC AMIDASE AND AN INHIBITOR OF T7 RNA POLYMERASE'''
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 [[Category: Single protein]]
 [[Category: Cheng, X.]]
-[[Category: hydrolase(acting on linear amides)]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 23:45:07 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:00:41 2008''