5d17: Difference between revisions
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<StructureSection load='5d17' size='340' side='right'caption='[[5d17]], [[Resolution|resolution]] 2.85Å' scene=''> | <StructureSection load='5d17' size='340' side='right'caption='[[5d17]], [[Resolution|resolution]] 2.85Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5d17]] is a 12 chain structure with sequence from [ | <table><tr><td colspan='2'>[[5d17]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D17 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5D17 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5d17 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d17 OCA], [https://pdbe.org/5d17 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5d17 RCSB], [https://www.ebi.ac.uk/pdbsum/5d17 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5d17 ProSAT]</span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/TNSE_ECOLX TNSE_ECOLX] TnsABC + TnsD promote high-frequency insertion of Tn7 into a specific target site known as att-Tn7 whereas TnsABC + TnsE promote low-frequency insertion into many different sites. | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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==See Also== | ==See Also== | ||
*[[Transposase|Transposase]] | *[[Transposase 3D structures|Transposase 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Escherichia coli]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Caron | [[Category: Caron JJ]] | ||
[[Category: Guarne | [[Category: Guarne A]] | ||
Revision as of 13:17, 21 June 2023
Structure of the C-terminal domain of TnsE at 2.85 resolutionStructure of the C-terminal domain of TnsE at 2.85 resolution
Structural highlights
FunctionTNSE_ECOLX TnsABC + TnsD promote high-frequency insertion of Tn7 into a specific target site known as att-Tn7 whereas TnsABC + TnsE promote low-frequency insertion into many different sites. Publication Abstract from PubMedThe bacterial transposon Tn7 facilitates horizontal transfer by directing transposition into actively replicating DNA with the element-encoded protein TnsE. Structural analysis of the C-terminal domain of wild-type TnsE identified a novel protein fold including a central V-shaped loop that toggles between two distinct conformations. The structure of a robust TnsE gain-of-activity variant has this loop locked in a single conformation, suggesting that conformational flexibility regulates TnsE activity. Structure-based analysis of a series of TnsE mutants relates transposition activity to DNA binding stability. Wild-type TnsE appears to naturally form an unstable complex with a target DNA, whereas mutant combinations required for large changes in transposition frequency and targeting stabilized this interaction. Collectively, our work unveils a unique structural proofreading mechanism where toggling between two conformations regulates target commitment by limiting the stability of target DNA engagement until an appropriate insertion site is identified. Conformational toggling controls target site choice for the heteromeric transposase element Tn7.,Shi Q, Straus MR, Caron JJ, Wang H, Chung YS, Guarne A, Peters JE Nucleic Acids Res. 2015 Sep 17. pii: gkv913. PMID:26384427[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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