5unk: Difference between revisions
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==NMR structure of the RED subdomain of the Sleeping Beauty transposase== | ==NMR structure of the RED subdomain of the Sleeping Beauty transposase== | ||
<StructureSection load='5unk' size='340' side='right'caption='[[5unk | <StructureSection load='5unk' size='340' side='right'caption='[[5unk]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5unk]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[5unk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oncorhynchus_mykiss Oncorhynchus mykiss]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UNK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5UNK FirstGlance]. <br> | ||
</td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5unk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5unk OCA], [https://pdbe.org/5unk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5unk RCSB], [https://www.ebi.ac.uk/pdbsum/5unk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5unk ProSAT]</span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A060ZAA1_ONCMY A0A060ZAA1_ONCMY] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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==See Also== | ==See Also== | ||
*[[Transposase|Transposase]] | *[[Transposase 3D structures|Transposase 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Oncorhynchus mykiss]] | ||
[[Category: Konnova | [[Category: Konnova TA]] | ||
[[Category: Nesmelova | [[Category: Nesmelova IV]] | ||
[[Category: Singer | [[Category: Singer CM]] | ||
Latest revision as of 13:26, 14 June 2023
NMR structure of the RED subdomain of the Sleeping Beauty transposaseNMR structure of the RED subdomain of the Sleeping Beauty transposase
Structural highlights
FunctionPublication Abstract from PubMedDNA transposons can be employed for stable gene transfer in vertebrates. The Sleeping Beauty (SB) DNA transposon has been recently adapted for human application and is being evaluated in clinical trials, however its molecular mechanism is not clear. SB transposition is catalyzed by the transposase enzyme, which is a multi-domain protein containing the catalytic and the DNA-binding domains. The DNA-binding domain of the SB transposase contains two structurally independent subdomains, PAI and RED. Recently, the structures of the catalytic domain and the PAI subdomain have been determined, however no structural information on the RED subdomain and its interactions with DNA has been available. Here, we used NMR spectroscopy to determine the solution structure of the RED subdomain and characterize its interactions with the transposon DNA. NMR solution structure of the RED subdomain of the Sleeping Beauty transposase.,Konnova TA, Singer CM, Nesmelova IV Protein Sci. 2017 Jun;26(6):1171-1181. doi: 10.1002/pro.3167. Epub 2017 Apr 2. PMID:28345263[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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