1l3e: Difference between revisions

Revision as of 23:29, 2 May 2008

NMR Structures of the HIF-1alpha CTAD/p300 CH1 Complex

OverviewOverview

Adaptation to hypoxia is mediated by transactivation of hypoxia-responsive genes by hypoxia-inducible factor-1 (HIF-1) in complex with the CBP and p300 transcriptional coactivators. We report the solution structure of the cysteine/histidine-rich 1 (CH1) domain of p300 bound to the C-terminal transactivation domain of HIF-1 alpha. CH1 has a triangular geometry composed of four alpha-helices with three intervening Zn(2+)-coordinating centers. CH1 serves as a scaffold for folding of the HIF-1 alpha C-terminal transactivation domain, which forms a vise-like clamp on the CH1 domain that is stabilized by extensive hydrophobic and polar interactions. The structure reveals the mechanism of specific recognition of p300 by HIF-1 alpha, and shows how HIF-1 alpha transactivation is regulated by asparagine hydroxylation.

About this StructureAbout this Structure

1L3E is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis for recruitment of CBP/p300 by hypoxia-inducible factor-1 alpha., Freedman SJ, Sun ZY, Poy F, Kung AL, Livingston DM, Wagner G, Eck MJ, Proc Natl Acad Sci U S A. 2002 Apr 16;99(8):5367-72. PMID:11959990 Page seeded by OCA on Fri May 2 23:29:40 2008

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OCA

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 [[Image:1l3e.gif|left|200px]]
- {{Structure
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-|GENE= hypoxia inducible factor-1 alpha ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), p300 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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-}}
 '''NMR Structures of the HIF-1alpha CTAD/p300 CH1 Complex'''
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 [[Category: Sun, Z J.]]
 [[Category: Wagner, G.]]
-[[Category: protein-protein complex]]
+[[Category: Protein-protein complex]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 23:29:40 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:57:27 2008''