1l3b: Difference between revisions

Revision as of 23:29, 2 May 2008

MT0146, THE PRECORRIN-6Y METHYLTRANSFERASE (CBIT) HOMOLOG FROM M. THERMOAUTOTROPHICUM, C2 SPACEGROUP W/ LONG CELL

OverviewOverview

The CbiT and CbiE enzymes participate in the biosynthesis of vitamin B12. They are fused together in some organisms to form a protein called CobL, which catalyzes two methylations and one decarboxylation on a precorrin intermediate. Because CbiE has sequence homology to canonical precorrin methyltransferases, CbiT was hypothesized to catalyze the decarboxylation. We herein present the crystal structure of MT0146, the CbiT homolog from Methanobacterium thermoautotrophicum. The protein shows structural similarity to Rossmann-like S-adenosyl-methionine-dependent methyltransferases, and our 1.9 A cocrystal structure shows that it binds S-adenosyl-methionine in standard geometry near a binding pocket that could accommodate a precorrin substrate. Therefore, MT0146/CbiT probably functions as a precorrin methyltransferase and represents the first enzyme identified with this activity that does not have the canonical precorrin methyltransferase fold.

About this StructureAbout this Structure

1L3B is a Single protein structure of sequence from Methanothermobacter thermautotrophicus. Full crystallographic information is available from OCA.

ReferenceReference

The crystal structure of MT0146/CbiT suggests that the putative precorrin-8w decarboxylase is a methyltransferase., Keller JP, Smith PM, Benach J, Christendat D, deTitta GT, Hunt JF, Structure. 2002 Nov;10(11):1475-87. PMID:12429089 Page seeded by OCA on Fri May 2 23:29:30 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1l3b.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1l3b |SIZE=350|CAPTION= <scene name='initialview01'>1l3b</scene>, resolution 2.65&Aring;
+The line below this paragraph, containing "STRUCTURE_1l3b", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE= MTH146 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=145262 Methanothermobacter thermautotrophicus])
+-->
-|DOMAIN=
+{{STRUCTURE_1l3b|  PDB=1l3b  |  SCENE=  }}
-|RELATEDENTRY=[[1kxz|1kxz]], [[1f38|1f38]], [[1l3c|1L3C]], [[1l3i|1L3I]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1l3b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l3b OCA], [http://www.ebi.ac.uk/pdbsum/1l3b PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1l3b RCSB]</span>
-}}
 '''MT0146, THE PRECORRIN-6Y METHYLTRANSFERASE (CBIT) HOMOLOG FROM M. THERMOAUTOTROPHICUM, C2 SPACEGROUP W/ LONG CELL'''
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 [[Category: Keller, J P.]]
 [[Category: Smith, P M.]]
-[[Category: deTitta, G.]]
+[[Category: DeTitta, G.]]
-[[Category: beta barrel]]
+[[Category: Beta barrel]]
-[[Category: decarboxylase]]
+[[Category: Decarboxylase]]
-[[Category: methyltransferase]]
+[[Category: Methyltransferase]]
-[[Category: rossmann fold]]
+[[Category: Rossmann fold]]
-[[Category: structural genomic]]
+[[Category: Structural genomic]]
-[[Category: structure-based function assignment]]
+[[Category: Structure-based function assignment]]
-[[Category: tetramer]]
+[[Category: Tetramer]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 23:29:30 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:57:23 2008''