1l2k: Difference between revisions

Revision as of 23:28, 2 May 2008

Neutron Structure Determination of Sperm Whale Met-Myoglobin at 1.5A Resolution.

OverviewOverview

From the first days of protein neutron structure determination sperm whale myoglobin was an object under investigation [Nature 224 (1969) 143, J. Mol. Biol. 220 (1991) 381]. Nevertheless myoglobin is still of interest [Proc. Natl. Acad. Sci. USA 97 (2000) 3872]. The feasibility of the monochromatic neutron diffractometer BIX-3 at the JRR-3M reactor at the JAERI [J. Phys. Chem. Solids 60 (1999) 1623], to collect high-resolution diffraction data in a relatively short time stimulated us to repeat the structural determination of myoglobin. The structure of metmyoglobin has been determined up to a resolution of 1.5 A. The hydrogen atoms were replaced in part, by deuterium soaking the crystals for more than 10 years in D(2)O. A refinement of all atoms has been performed including the refinement of individual mean square displacements and occupancies of the exchangeable protons in backbone hydrogen bonds. A method is described to show clear negative scattering densities of the H atoms. Water molecules within the protein and on the molecule surface are shown. The exchangeability of H atoms is correlated with structural distribution and flexibility.

About this StructureAbout this Structure

1L2K is a Single protein structure of sequence from Physeter catodon. Full crystallographic information is available from OCA.

ReferenceReference

Hydrogen and deuterium in myoglobin as seen by a neutron structure determination at 1.5 A resolution., Ostermann A, Tanaka I, Engler N, Niimura N, Parak FG, Biophys Chem. 2002 Mar 28;95(3):183-93. PMID:12062378 Page seeded by OCA on Fri May 2 23:28:10 2008

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OCA

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 [[Image:1l2k.gif|left|200px]]
- {{Structure
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-}}
 '''Neutron Structure Determination of Sperm Whale Met-Myoglobin at 1.5A Resolution.'''
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 [[Category: Parak, F G.]]
 [[Category: Tanaka, I.]]
-[[Category: heme protein]]
+[[Category: Heme protein]]
-[[Category: hydration structure]]
+[[Category: Hydration structure]]
-[[Category: hydrogen atom]]
+[[Category: Hydrogen atom]]
-[[Category: neutron structure]]
+[[Category: Neutron structure]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 23:28:10 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:57:05 2008''