5c21: Difference between revisions
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<StructureSection load='5c21' size='340' side='right'caption='[[5c21]], [[Resolution|resolution]] 2.50Å' scene=''> | <StructureSection load='5c21' size='340' side='right'caption='[[5c21]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5c21]] is a 2 chain structure with sequence from [ | <table><tr><td colspan='2'>[[5c21]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C21 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5C21 FirstGlance]. <br> | ||
</td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5c21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c21 OCA], [https://pdbe.org/5c21 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5c21 RCSB], [https://www.ebi.ac.uk/pdbsum/5c21 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5c21 ProSAT]</span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/HLYDC_ECOLX HLYDC_ECOLX] Involved in the transport of hemolysin A. | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Escherichia coli]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Ha | [[Category: Ha NC]] | ||
[[Category: Kim | [[Category: Kim JS]] | ||
[[Category: Yoon | [[Category: Yoon BY]] | ||
Revision as of 09:16, 7 June 2023
Crystal structure of native HlyD from E. coliCrystal structure of native HlyD from E. coli
Structural highlights
FunctionHLYDC_ECOLX Involved in the transport of hemolysin A. Publication Abstract from PubMedThe protein toxin HlyA of Escherichia coli is exported without a periplasmic intermediate by the type I secretion system (T1SS). The T1SS is composed of an inner membrane ABC transporter HlyB, an outer-membrane channel protein TolC, and a membrane fusion protein HlyD. However, the assembly of the T1SS remains to be elucidated. In this study, we determine the crystal structure of a part of the C-terminal periplasmic domain of HlyD. The long alpha-helical domain consisting of three alpha helices and a lipoyl domain was identified in the crystal structure. Based on the HlyD structure, we modeled the hexameric assembly of HlyD with a long alpha-helical barrel, which formed a complex with TolC in an intermeshing cogwheel-to-cogwheel manner, as observed in tripartite RND-type drug efflux pumps. These observations provide a structural blueprint for understanding the type I secretion system in pathogenic Gram-negative bacteria. Crystal Structure of a Soluble Fragment of the Membrane Fusion Protein HlyD in a Type I Secretion System of Gram-Negative Bacteria.,Kim JS, Song S, Lee M, Lee S, Lee K, Ha NC Structure. 2016 Jan 22. pii: S0969-2126(16)00005-8. doi:, 10.1016/j.str.2015.12.012. PMID:26833388[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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