1bsu: Difference between revisions
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<StructureSection load='1bsu' size='340' side='right'caption='[[1bsu]], [[Resolution|resolution]] 2.00Å' scene=''> | <StructureSection load='1bsu' size='340' side='right'caption='[[1bsu]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1bsu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[1bsu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BSU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BSU FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5CM:5-METHYL-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>5CM</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bsu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bsu OCA], [https://pdbe.org/1bsu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bsu RCSB], [https://www.ebi.ac.uk/pdbsum/1bsu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bsu ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bsu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bsu OCA], [https://pdbe.org/1bsu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bsu RCSB], [https://www.ebi.ac.uk/pdbsum/1bsu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bsu ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/T2E5_ECOLX T2E5_ECOLX] Recognizes the double-stranded sequence GATATC and cleaves after T-3. | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Escherichia coli]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Martin AM]] | |||
[[Category: Martin | [[Category: Perona JJ]] | ||
[[Category: Perona | |||
Revision as of 09:04, 31 May 2023
STRUCTURAL AND ENERGETIC ORIGINS OF INDIRECT READOUT IN SITE-SPECIFIC DNA CLEAVAGE BY A RESTRICTION ENDONUCLEASESTRUCTURAL AND ENERGETIC ORIGINS OF INDIRECT READOUT IN SITE-SPECIFIC DNA CLEAVAGE BY A RESTRICTION ENDONUCLEASE
Structural highlights
FunctionT2E5_ECOLX Recognizes the double-stranded sequence GATATC and cleaves after T-3. Publication Abstract from PubMedSpecific recognition by EcoRV endonuclease of its cognate, sharply bent GATATC site at the center TA step occurs solely via hydrophobic interaction with thymine methyl groups. Mechanistic kinetic analyses of base analog-substituted DNAs at this position reveal that direct readout provides 5 kcal mol(-1) toward specificity, with an additional 6-10 kcal mol(-1) arising from indirect readout. Crystal structures of several base analog complexes show that the major-groove hydrophobic contacts are crucial to forming required divalent metal-binding sites, and that indirect readout operates in part through the sequence-dependent free-energy cost of unstacking the center base-pair step of the DNA. Structural and energetic origins of indirect readout in site-specific DNA cleavage by a restriction endonuclease.,Martin AM, Sam MD, Reich NO, Perona JJ Nat Struct Biol. 1999 Mar;6(3):269-77. PMID:10074946[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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