5aij: Difference between revisions

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<StructureSection load='5aij' size='340' side='right'caption='[[5aij]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
<StructureSection load='5aij' size='340' side='right'caption='[[5aij]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5aij]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AIJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AIJ FirstGlance]. <br>
<table><tr><td colspan='2'>[[5aij]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AIJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AIJ FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5aij FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aij OCA], [http://pdbe.org/5aij PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5aij RCSB], [http://www.ebi.ac.uk/pdbsum/5aij PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5aij ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5aij FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aij OCA], [https://pdbe.org/5aij PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5aij RCSB], [https://www.ebi.ac.uk/pdbsum/5aij PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5aij ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/LPAKS_PSEAE LPAKS_PSEAE] Alkylsulfatase that cleaves both short and medium-length alkylsulfates, including the widely used detergent sodium dodecyl sulfate (SDS) (PubMed:16684886). Acts in vitro on primary alkylsulfates such as decyl sulfate, octyl sulfate and hexyl sulfate, but not on sulfated sugars and arylsulfates (PubMed:16684886, PubMed:23061549). The hydrolysis of SDS allows the bacterium to use SDS as a sole carbon or sulfur source (PubMed:16684886). In addition, contributes to the in vivo virulence of P.aeruginosa by playing a central role in the degradation of mucin, a glycoprotein that is the primary component of the mucus overlaying the host epithelial tissues (PubMed:31907968). Plays a role in the degradation of both protein and carbohydrate moieties in mucin via the release of sulfate from mucin, D-galactose 6-sulfate (Gal-6S) and N-acetyl-D-glucosamine 6-sulfate (GlcNAc-6S) (PubMed:31907968).<ref>PMID:16684886</ref> <ref>PMID:23061549</ref> <ref>PMID:31907968</ref>


==See Also==
==See Also==
*[[Antibody 3D structures|Antibody 3D structures]]
*[[Antibody 3D structures|Antibody 3D structures]]
*[[Sulfatase 3D structures|Sulfatase 3D structures]]
*[[Sulfatase 3D structures|Sulfatase 3D structures]]
*[[3D structures of non-human antibody|3D structures of non-human antibody]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Flores-Hernandez, E]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Jakoncic, J]]
[[Category: De la Mora E]]
[[Category: Mora, E De la]]
[[Category: Flores-Hernandez E]]
[[Category: Moreno, A]]
[[Category: Jakoncic J]]
[[Category: Sanchez-Puig, N]]
[[Category: Moreno A]]
[[Category: Stojanoff, V]]
[[Category: Sanchez-Puig N]]
[[Category: Hydrolase]]
[[Category: Stojanoff V]]
[[Category: Sdsa sulfatase polymorph]]

Revision as of 08:02, 25 May 2023

P. aeruginosa SdsA hexagonal polymorphP. aeruginosa SdsA hexagonal polymorph

Structural highlights

5aij is a 1 chain structure with sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LPAKS_PSEAE Alkylsulfatase that cleaves both short and medium-length alkylsulfates, including the widely used detergent sodium dodecyl sulfate (SDS) (PubMed:16684886). Acts in vitro on primary alkylsulfates such as decyl sulfate, octyl sulfate and hexyl sulfate, but not on sulfated sugars and arylsulfates (PubMed:16684886, PubMed:23061549). The hydrolysis of SDS allows the bacterium to use SDS as a sole carbon or sulfur source (PubMed:16684886). In addition, contributes to the in vivo virulence of P.aeruginosa by playing a central role in the degradation of mucin, a glycoprotein that is the primary component of the mucus overlaying the host epithelial tissues (PubMed:31907968). Plays a role in the degradation of both protein and carbohydrate moieties in mucin via the release of sulfate from mucin, D-galactose 6-sulfate (Gal-6S) and N-acetyl-D-glucosamine 6-sulfate (GlcNAc-6S) (PubMed:31907968).[1] [2] [3]

See Also

References

  1. ↑ Hagelueken G, Adams TM, Wiehlmann L, Widow U, Kolmar H, Tummler B, Heinz DW, Schubert WD. The crystal structure of SdsA1, an alkylsulfatase from Pseudomonas aeruginosa, defines a third class of sulfatases. Proc Natl Acad Sci U S A. 2006 May 16;103(20):7631-6. Epub 2006 May 9. PMID:16684886
  2. ↑ Knaus T, Schober M, Kepplinger B, Faccinelli M, Pitzer J, Faber K, Macheroux P, Wagner U. Structure and mechanism of an inverting alkylsulfatase from Pseudomonas sp. DSM6611 specific for secondary alkyl sulfates. FEBS J. 2012 Dec;279(23):4374-84. doi: 10.1111/febs.12027. Epub 2012 Nov 7. PMID:23061549 doi:http://dx.doi.org/10.1111/febs.12027
  3. ↑ Kida Y, Yamamoto T, Kuwano K. SdsA1, a secreted sulfatase, contributes to the in vivo virulence of Pseudomonas aeruginosa in mice. Microbiol Immunol. 2020 Apr;64(4):280-295. PMID:31907968 doi:10.1111/1348-0421.12772

5aij, resolution 1.95Å

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