1kvy: Difference between revisions

Revision as of 23:14, 2 May 2008

CARBOXYLIC ESTER HYDROLASE, SINGLE MUTANT D49E COORDINATED TO CALCIUM

OverviewOverview

Crystal structures of the active-site mutants D99A and H48Q and the calcium-loop mutant D49E of bovine phospholipase A2 have been determined at around 1.9 A resolution. The D99A mutant is isomorphous to the orthorhombic recombinant enzyme, space group P212121. The H48Q and the calcium-loop mutant D49E are isomorphous to the trigonal recombinant enzyme, space group P3121. The two active-site mutants show no major structural perturbations. The structural water is absent in D99A and, therefore, the hydrogen-bonding scheme is changed. In H48Q, the catalytic water is present and hydrogen bonded to Gln48 N, but the second water found in native His48 is absent. In the calcium-loop mutant D49E, the two water molecules forming the pentagonal bipyramid around calcium are absent and only one O atom of the Glu49 carboxylate group is coordinated to calcium, resulting in only four ligands.

About this StructureAbout this Structure

1KVY is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

ReferenceReference

Structures of the catalytic site mutants D99A and H48Q and the calcium-loop mutant D49E of phospholipase A2., Sekar K, Biswas R, Li Y, Tsai M, Sundaralingam M, Acta Crystallogr D Biol Crystallogr. 1999 Feb;55(Pt 2):443-7. PMID:10089353 Page seeded by OCA on Fri May 2 23:14:03 2008

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OCA

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 [[Image:1kvy.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1kvy |SIZE=350|CAPTION= <scene name='initialview01'>1kvy</scene>, resolution 1.9&Aring;
+The line below this paragraph, containing "STRUCTURE_1kvy", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= MATURE PLA2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 Bos taurus])
+-->
-|DOMAIN=
+{{STRUCTURE_1kvy|  PDB=1kvy  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kvy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kvy OCA], [http://www.ebi.ac.uk/pdbsum/1kvy PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1kvy RCSB]</span>
-}}
 '''CARBOXYLIC ESTER HYDROLASE, SINGLE MUTANT D49E COORDINATED TO CALCIUM'''
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 Structures of the catalytic site mutants D99A and H48Q and the calcium-loop mutant D49E of phospholipase A2., Sekar K, Biswas R, Li Y, Tsai M, Sundaralingam M, Acta Crystallogr D Biol Crystallogr. 1999 Feb;55(Pt 2):443-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10089353 10089353]
 [[Category: Bos taurus]]
-[[Category: Phospholipase A(2)]]
 [[Category: Single protein]]
 [[Category: Sundaralingam, M.]]
-[[Category: carboxylic ester hydrolase]]
+[[Category: Carboxylic ester hydrolase]]
-[[Category: enzyme]]
+[[Category: Enzyme]]
-[[Category: hydrolase]]
+[[Category: Hydrolase]]
-[[Category: mutant]]
+[[Category: Mutant]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 23:14:03 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:54:18 2008''