4zvf: Difference between revisions

<StructureSection load='4zvf' size='340' side='right' caption='[[4zvf]], [[Resolution|resolution]] 1.15&Aring;' scene=''>

<table><tr><td colspan='2'>[[4zvf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZVF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZVF FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GAV:GUANOSINE-5-RP-ALPHA-THIO-TRIPHOSPHATE'>GAV</scene></td></tr>

<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Diguanylate_cyclase Diguanylate cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.65 2.7.7.65] </span></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zvf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zvf OCA], [http://pdbe.org/4zvf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zvf RCSB], [http://www.ebi.ac.uk/pdbsum/4zvf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zvf ProSAT]</span></td></tr>

[[http://www.uniprot.org/uniprot/DOSC_ECOLI DOSC_ECOLI]] Globin-coupled heme-based oxygen sensor protein displaying diguanylate cyclase (DGC) activity in response to oxygen availability. Thus, catalyzes the synthesis of cyclic diguanylate (c-di-GMP) via the condensation of 2 GTP molecules. Is involved in the modulation of intracellular c-di-GMP levels, in association with DosP which catalyzes the degradation of c-di-GMP (PDE activity). Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria. DosC regulates biofilm formation through the oxygen-dependent activation of the csgBAC operon, which encodes curli structural subunits, while not affecting the expression of the regulatory operon csgDEFG. DosC, but not the other DGCs in E.coli, also promotes the production of the exopolysaccharide poly-N-acetylglucosamine (PNAG) through up-regulation of the expression of the PNAG biosynthetic pgaABCD operon, independently of CsrA.  Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain TOB1, a fecal isolate) overexpression leads to an increase in cellulose production.  

[[Category: Diguanylate cyclase]]

[[Category: Ecoli]]

[[Category: Barends, T R.M]]

[[Category: Schlichting, I]]

[[Category: Tarnawski, M]]

[[Category: Signaling protein]]