1ksy: Difference between revisions

Revision as of 23:08, 2 May 2008

Crystal Structures of Two Intermediates in the Assembly of the Papillomavirus Replication Initiation Complex

OverviewOverview

Initiation of DNA replication of the papillomavirus genome is a multi-step process involving the sequential loading of viral E1 protein subunits onto the origin of replication. Here we have captured structural snapshots of two sequential steps in the assembly process. Initially, an E1 dimer binds to adjacent major grooves on one face of the double helix; a second dimer then binds to another face of the helix. Each E1 monomer has two DNA-binding modules: a DNA-binding loop, which binds to one DNA strand and a DNA-binding helix, which binds to the opposite strand. The nature of DNA binding suggests a mechanism for the transition between double- and single-stranded DNA binding that is implicit in the progression to a functional helicase.

About this StructureAbout this Structure

1KSY is a Single protein structure of sequence from Bovine papillomavirus. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structures of two intermediates in the assembly of the papillomavirus replication initiation complex., Enemark EJ, Stenlund A, Joshua-Tor L, EMBO J. 2002 Mar 15;21(6):1487-96. PMID:11889054 Page seeded by OCA on Fri May 2 23:08:09 2008

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OCA

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 [[Image:1ksy.gif|left|200px]]
- {{Structure
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ksy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ksy OCA], [http://www.ebi.ac.uk/pdbsum/1ksy PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ksy RCSB]</span>
-}}
 '''Crystal Structures of Two Intermediates in the Assembly of the Papillomavirus Replication Initiation Complex'''
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 [[Category: Joshua-Tor, L.]]
 [[Category: Stenlund, A.]]
-[[Category: dna-binding domain]]
+[[Category: Dna-binding domain]]
-[[Category: helicase]]
+[[Category: Helicase]]
-[[Category: initiator protein]]
+[[Category: Initiator protein]]
-[[Category: papillomavirus]]
+[[Category: Papillomavirus]]
-[[Category: replication]]
+[[Category: Replication]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 23:08:09 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:53:06 2008''