1ksk: Difference between revisions

Revision as of 23:07, 2 May 2008

STRUCTURE OF RSUA

OverviewOverview

In Escherichia coli, the pseudouridine synthase RsuA catalyzes formation of pseudouridine (psi) at position 516 in 16S rRNA during assembly of the 30S ribosomal subunit. We have determined the crystal structure of RsuA bound to uracil at 2.0 A resolution and to uridine 5'-monophosphate (UMP) at 2.65 A resolution. RsuA consists of an N-terminal domain connected by an extended linker to the central and C-terminal domains. Uracil and UMP bind in a cleft between the central and C-terminal domains near the catalytic residue Asp 102. The N-terminal domain shows structural similarity to the ribosomal protein S4. Despite only 15% amino acid identity, the other two domains are structurally similar to those of the tRNA-specific psi-synthase TruA, including the position of the catalytic Asp. Our results suggest that all four families of pseudouridine synthases share the same fold of their catalytic domain(s) and uracil-binding site.

About this StructureAbout this Structure

1KSK is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Structure of the 16S rRNA pseudouridine synthase RsuA bound to uracil and UMP., Sivaraman J, Sauve V, Larocque R, Stura EA, Schrag JD, Cygler M, Matte A, Nat Struct Biol. 2002 May;9(5):353-8. PMID:11953756 Page seeded by OCA on Fri May 2 23:07:15 2008

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OCA

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 [[Image:1ksk.gif|left|200px]]
- {{Structure
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-|LIGAND= <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=URA:URACIL'>URA</scene>
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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Pseudouridylate_synthase Pseudouridylate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.70 4.2.1.70] </span>
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-|RELATEDENTRY=[[1ksl|1KSL]], [[1ksv|1KSV]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ksk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ksk OCA], [http://www.ebi.ac.uk/pdbsum/1ksk PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ksk RCSB]</span>
-}}
 '''STRUCTURE OF RSUA'''
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 [[Category: Sivaraman, J.]]
 [[Category: Stura, E A.]]
-[[Category: crystal structure]]
+[[Category: Crystal structure]]
-[[Category: pseudouridine synthase]]
+[[Category: Pseudouridine synthase]]
-[[Category: rsua]]
+[[Category: Rsua]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 23:07:15 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:52:58 2008''