4z7l: Difference between revisions
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==Crystal structure of Cas6b== | ==Crystal structure of Cas6b== | ||
<StructureSection load='4z7l' size='340' side='right' caption='[[4z7l]], [[Resolution|resolution]] 3.50Å' scene=''> | <StructureSection load='4z7l' size='340' side='right'caption='[[4z7l]], [[Resolution|resolution]] 3.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4z7l]] is a 9 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4z7l]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanococcus_maripaludis Methanococcus maripaludis] and [https://en.wikipedia.org/wiki/Methanococcus_maripaludis_C5 Methanococcus maripaludis C5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z7L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Z7L FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4z7l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z7l OCA], [https://pdbe.org/4z7l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4z7l RCSB], [https://www.ebi.ac.uk/pdbsum/4z7l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4z7l ProSAT]</span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/A4FXZ3_METM5 A4FXZ3_METM5] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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</div> | </div> | ||
<div class="pdbe-citations 4z7l" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 4z7l" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[CRISPR subtype I-B|CRISPR subtype I-B]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Methanococcus maripaludis]] | ||
[[Category: | [[Category: Methanococcus maripaludis C5]] | ||
[[Category: | [[Category: Li H]] | ||
Revision as of 10:42, 10 May 2023
Crystal structure of Cas6bCrystal structure of Cas6b
Structural highlights
FunctionPublication Abstract from PubMedA subclass of recently discovered CRISPR repeat RNA in bacteria contains minimally recognizable structural features that facilitate an unknown mechanism of recognition and processing by the Cas6 family of endoribonucleases. Cocrystal structures of Cas6 from Methanococcus maripaludis (MmCas6b) bound with its repeat RNA revealed a dual site binding structure and a cleavage site conformation poised for phosphodiester bond breakage. Two non-interacting MmCas6b bind to two separate AAYAA motifs within the same repeat, one distal and one adjacent to the cleavage site. This bound structure potentially competes with a stable but non-productive RNA structure. At the cleavage site, MmCas6b supplies a base pair mimic to stabilize a short 2 base pair stem immediately upstream of the scissile phosphate. Complementary biochemical analyses support the dual-AAYAA binding model and a critical role of the protein-RNA base pair mimic. Our results reveal a previously unknown method of processing non-stem-loop CRISPR RNA by Cas6. A Non-Stem-Loop CRISPR RNA Is Processed by Dual Binding Cas6.,Shao Y, Richter H, Sun S, Sharma K, Urlaub H, Randau L, Li H Structure. 2016 Apr 5;24(4):547-554. doi: 10.1016/j.str.2016.02.009. Epub 2016, Mar 17. PMID:26996962[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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