1krv: Difference between revisions

Revision as of 23:05, 2 May 2008

Galactoside Acetyltransferase in Complex with CoA and PNP-beta-Gal

OverviewOverview

The galactoside acetyltransferase (thiogalactoside transacetylase) of Escherichia coli (GAT, LacA, EC 2.3.1.18) is a gene product of the classical lac operon. GAT may assist cellular detoxification by acetylating nonmetabolizable pyranosides, thereby preventing their reentry into the cell. The structure of GAT has been solved in binary complexes with acetyl-CoA or CoA and in ternary complexes with CoA and the nonphysiological acceptor substrates isopropyl beta-D-thiogalactoside (IPTG) or p-nitrophenyl beta-D-galactopyranoside (PNPbetaGal). A hydrophobic cleft that binds the thioisopropyl and p-nitrophenyl aglycones of IPTG and PNPbetaGal may discriminate against substrates with hydrophilic substituents at this position, such as lactose, or inducers of the lac operon. An extended loop projecting from the left-handed parallel beta helix domain contributes His115, which is in position to facilitate attack of the C6-hydroxyl group of the substrate on the thioester.

About this StructureAbout this Structure

1KRV is a Single protein structure of sequence from Escherichia coli. The following page contains interesting information on the relation of 1KRV with [lac Repressor]. Full crystallographic information is available from OCA.

ReferenceReference

Structure of the lac operon galactoside acetyltransferase., Wang XG, Olsen LR, Roderick SL, Structure. 2002 Apr;10(4):581-8. PMID:11937062 Page seeded by OCA on Fri May 2 23:05:42 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1krv.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1krv |SIZE=350|CAPTION= <scene name='initialview01'>1krv</scene>, resolution 2.80&Aring;
+The line below this paragraph, containing "STRUCTURE_1krv", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=147:1-O-[P-NITROPHENYL]-BETA-D-GALACTOPYRANOSE'>147</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Galactoside_O-acetyltransferase Galactoside O-acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.18 2.3.1.18] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= lacA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+-->
-|DOMAIN=
+{{STRUCTURE_1krv|  PDB=1krv  |  SCENE=  }}
-|RELATEDENTRY=[[1kqa|1KQA]], [[1krr|1KRR]], [[1kru|1KRU]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1krv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1krv OCA], [http://www.ebi.ac.uk/pdbsum/1krv PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1krv RCSB]</span>
-}}
 '''Galactoside Acetyltransferase in Complex with CoA and PNP-beta-Gal'''
@@ Line 26: / Line 23: @@
 [[Category: Galactoside O-acetyltransferase]]
 [[Category: Single protein]]
-[[Category: lac Repressor]]
+[[Category: Lac Repressor]]
 [[Category: Olsen, L R.]]
 [[Category: Roderick, S L.]]
 [[Category: Wang, X G.]]
-[[Category: left-handed parallel beta helix]]
+[[Category: Left-handed parallel beta helix]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 23:05:42 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:52:43 2008''