4yuc: Difference between revisions
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<StructureSection load='4yuc' size='340' side='right'caption='[[4yuc]], [[Resolution|resolution]] 1.31Å' scene=''> | <StructureSection load='4yuc' size='340' side='right'caption='[[4yuc]], [[Resolution|resolution]] 1.31Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4yuc]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YUC OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[4yuc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Corallococcus_coralloides Corallococcus coralloides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YUC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YUC FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4yuc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yuc OCA], [https://pdbe.org/4yuc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4yuc RCSB], [https://www.ebi.ac.uk/pdbsum/4yuc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4yuc ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/D7RK32_CORCK D7RK32_CORCK] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Corallococcus coralloides]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Stehle | [[Category: Stehle T]] | ||
[[Category: Vilstrup | [[Category: Vilstrup J]] | ||
[[Category: Zocher | [[Category: Zocher G]] | ||
Revision as of 10:26, 10 May 2023
Crystal Structure of CorB derivatized with S-(2-acetamidoethyl) 4-methyl-3-oxohexanethioateCrystal Structure of CorB derivatized with S-(2-acetamidoethyl) 4-methyl-3-oxohexanethioate
Structural highlights
FunctionPublication Abstract from PubMedCorallopyronin A is a polyketide derived from the myxobacterium Corallococcus coralloides with potent antibiotic features. The gene cluster responsible for the biosynthesis of corallopyronin A has been described recently, and it was proposed that CorB acts as a ketosynthase to interconnect two polyketide chains in a rare head-to-head condensation reaction. We determined the structure of CorB, the interconnecting polyketide synthase, to high resolution and found that CorB displays a thiolase fold. Site-directed mutagenesis showed that the catalytic triad consisting of a cysteine, a histidine and an asparagine is crucial for catalysis, and that this triad shares similarities with the triad found in HMG-CoA synthases. We synthesized a substrate mimic to derivatize purified CorB and confirmed substrate attachment by ESI-MS. Structural analysis of the complex yielded an electron density-based model for the polyketide chain and showed that the unusually wide, T-shaped active site is able to accommodate two polyketides simultaneously. Our structural analysis provides a platform for understanding the unusual head-to-head polyketide-interconnecting reaction catalyzed by CorB. Structural basis of head to head polyketide fusion by CorB.,Zocher G, Vilstrup J, Heine D, Hallab A, Goralski E, Hertweck C, Stahl M, Schaberle TF, Stehle T Chem Sci. 2015 Nov 13;6(11):6525-6536. doi: 10.1039/c5sc02488a. Epub 2015 Aug 6. PMID:28757960[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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