8oh4: Difference between revisions
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==Subtomogram averaging structure of cofilactin filament inside microtubule lumen of Drosophila S2 cell protrusion.== | |||
<StructureSection load='8oh4' size='340' side='right'caption='[[8oh4]], [[Resolution|resolution]] 16.50Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8oh4]] is a 14 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila Drosophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8OH4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8OH4 FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8oh4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8oh4 OCA], [https://pdbe.org/8oh4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8oh4 RCSB], [https://www.ebi.ac.uk/pdbsum/8oh4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8oh4 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/ACT1_DROME ACT1_DROME] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Multiple isoforms are involved in various cellular functions such as cytoskeleton structure, cell mobility, chromosome movement and muscle contraction. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Cytoplasmic microtubules are tubular polymers that can harbor small proteins or filaments inside their lumen. The identity of these objects and what causes their accumulation has not been conclusively established. Here, we used cryogenic electron tomography (cryoET) of Drosophila S2 cell protrusions and found filaments inside the microtubule lumen, which resemble those reported recently in human HAP1 cells. The frequency of these filaments increased upon inhibition of the sarco/endoplasmic reticulum Ca (2+) ATPase (SERCA) with the small-molecule drug thapsigargin. Subtomogram averaging showed that the luminal filaments adopt a helical structure reminiscent of cofilin-bound actin (cofilactin). Consistent with this, cofilin was activated in cells under the same conditions that increased luminal filament occurrence. Furthermore, RNAi knock-down of cofilin reduced the frequency of luminal filaments with cofilactin morphology. These results suggest that cofilin activation stimulates its accumulation on actin filaments inside the microtubule lumen. | |||
CryoET shows cofilactin filaments inside the microtubule lumen.,Santos CV, Rogers SL, Carter AP bioRxiv. 2023 Mar 31:2023.03.31.535077. doi: 10.1101/2023.03.31.535077. Preprint. PMID:37034688<ref>PMID:37034688</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 8oh4" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Drosophila]] | |||
[[Category: Large Structures]] | |||
[[Category: Carter AP]] | |||
[[Category: Ventura Santos C]] | |||
Revision as of 09:58, 10 May 2023
Subtomogram averaging structure of cofilactin filament inside microtubule lumen of Drosophila S2 cell protrusion.Subtomogram averaging structure of cofilactin filament inside microtubule lumen of Drosophila S2 cell protrusion.
Structural highlights
FunctionACT1_DROME Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Multiple isoforms are involved in various cellular functions such as cytoskeleton structure, cell mobility, chromosome movement and muscle contraction. Publication Abstract from PubMedCytoplasmic microtubules are tubular polymers that can harbor small proteins or filaments inside their lumen. The identity of these objects and what causes their accumulation has not been conclusively established. Here, we used cryogenic electron tomography (cryoET) of Drosophila S2 cell protrusions and found filaments inside the microtubule lumen, which resemble those reported recently in human HAP1 cells. The frequency of these filaments increased upon inhibition of the sarco/endoplasmic reticulum Ca (2+) ATPase (SERCA) with the small-molecule drug thapsigargin. Subtomogram averaging showed that the luminal filaments adopt a helical structure reminiscent of cofilin-bound actin (cofilactin). Consistent with this, cofilin was activated in cells under the same conditions that increased luminal filament occurrence. Furthermore, RNAi knock-down of cofilin reduced the frequency of luminal filaments with cofilactin morphology. These results suggest that cofilin activation stimulates its accumulation on actin filaments inside the microtubule lumen. CryoET shows cofilactin filaments inside the microtubule lumen.,Santos CV, Rogers SL, Carter AP bioRxiv. 2023 Mar 31:2023.03.31.535077. doi: 10.1101/2023.03.31.535077. Preprint. PMID:37034688[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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