8gh9: Difference between revisions

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-'''Unreleased structure'''
-The entry 8gh9 is ON HOLD
+==Cryo-EM structure of hSlo1 in total membrane vesicles==
+<StructureSection load='8gh9' size='340' side='right'caption='[[8gh9]], [[Resolution|resolution]] 3.80&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[8gh9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8GH9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8GH9 FirstGlance]. <br>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8gh9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8gh9 OCA], [https://pdbe.org/8gh9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8gh9 RCSB], [https://www.ebi.ac.uk/pdbsum/8gh9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8gh9 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/KCMA1_HUMAN KCMA1_HUMAN]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Integral membrane protein structure determination traditionally requires extraction from cell membranes using detergents or polymers. Here, we describe the isolation and structure determination of proteins in membrane vesicles derived directly from cells. Structures of the ion channel Slo1 from total cell membranes and from cell plasma membranes were determined at 3.8 A and 2.7 A resolution, respectively. The plasma membrane environment stabilizes Slo1, revealing an alteration of global helical packing, polar lipid, and cholesterol interactions that stabilize previously unresolved regions of the channel and an additional ion binding site in the Ca(2+) regulatory domain. The two methods presented enable structural analysis of both internal and plasma membrane proteins without disrupting weakly interacting proteins, lipids, and cofactors that are essential to biological function.
-Authors:
+Membrane protein isolation and structure determination in cell-derived membrane vesicles.,Tao X, Zhao C, MacKinnon R Proc Natl Acad Sci U S A. 2023 May 2;120(18):e2302325120. doi: , 10.1073/pnas.2302325120. Epub 2023 Apr 25. PMID:37098056<ref>PMID:37098056</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 8gh9" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Homo sapiens]]
+[[Category: Large Structures]]
+[[Category: MacKinnon R]]
+[[Category: Tao X]]
+[[Category: Zhao C]]