4ysq: Difference between revisions
No edit summary |
No edit summary |
||
| Line 3: | Line 3: | ||
<StructureSection load='4ysq' size='340' side='right'caption='[[4ysq]], [[Resolution|resolution]] 1.50Å' scene=''> | <StructureSection load='4ysq' size='340' side='right'caption='[[4ysq]], [[Resolution|resolution]] 1.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4ysq]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4ysq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_thermodenitrificans_NG80-2 Geobacillus thermodenitrificans NG80-2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YSQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YSQ FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ysq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ysq OCA], [https://pdbe.org/4ysq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ysq RCSB], [https://www.ebi.ac.uk/pdbsum/4ysq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ysq ProSAT]</span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/A4IL26_GEOTN A4IL26_GEOTN] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
| Line 25: | Line 25: | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Geobacillus thermodenitrificans NG80-2]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Diederichs | [[Category: Diederichs K]] | ||
[[Category: Fukuda | [[Category: Fukuda Y]] | ||
[[Category: Hatsui | [[Category: Hatsui T]] | ||
[[Category: Hirata | [[Category: Hirata K]] | ||
[[Category: Inoue | [[Category: Inoue T]] | ||
[[Category: Iwata | [[Category: Iwata S]] | ||
[[Category: Joti | [[Category: Joti Y]] | ||
[[Category: Kameshima | [[Category: Kameshima T]] | ||
[[Category: Matsumura | [[Category: Matsumura H]] | ||
[[Category: Mizohata | [[Category: Mizohata E]] | ||
[[Category: Nakane | [[Category: Nakane T]] | ||
[[Category: Nango | [[Category: Nango E]] | ||
[[Category: Nureki | [[Category: Nureki O]] | ||
[[Category: Song | [[Category: Song C]] | ||
[[Category: Sugahara | [[Category: Sugahara M]] | ||
[[Category: Suzuki | [[Category: Suzuki M]] | ||
[[Category: Tono | [[Category: Tono K]] | ||
[[Category: Tse | [[Category: Tse KM]] | ||
[[Category: Yabashi | [[Category: Yabashi M]] | ||
Revision as of 11:29, 3 May 2023
Structure of copper nitrite reductase from Geobacillus thermodenitrificans - 8.38 MGyStructure of copper nitrite reductase from Geobacillus thermodenitrificans - 8.38 MGy
Structural highlights
FunctionPublication Abstract from PubMedSerial femtosecond crystallography (SFX) has enabled the damage-free structural determination of metalloenzymes and filled the gaps of our knowledge between crystallographic and spectroscopic data. Crystallographers, however, scarcely know whether the rising technique provides truly new structural insights into mechanisms of metalloenzymes partly because of limited resolutions. Copper nitrite reductase (CuNiR), which converts nitrite to nitric oxide in denitrification, has been extensively studied by synchrotron radiation crystallography (SRX). Although catalytic Cu (Type 2 copper (T2Cu)) of CuNiR had been suspected to tolerate X-ray photoreduction, we here showed that T2Cu in the form free of nitrite is reduced and changes its coordination structure in SRX. Moreover, we determined the completely oxidized CuNiR structure at 1.43 A resolution with SFX. Comparison between the high-resolution SFX and SRX data revealed the subtle structural change of a catalytic His residue by X-ray photoreduction. This finding, which SRX has failed to uncover, provides new insight into the reaction mechanism of CuNiR. Redox-coupled structural changes in nitrite reductase revealed by serial femtosecond and microfocus crystallography.,Fukuda Y, Tse KM, Suzuki M, Diederichs K, Hirata K, Nakane T, Sugahara M, Nango E, Tono K, Joti Y, Kameshima T, Song C, Hatsui T, Yabashi M, Nureki O, Matsumura H, Inoue T, Iwata S, Mizohata E J Biochem. 2016 Jan 14. pii: mvv133. PMID:26769972[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
|
| ||||||||||||||||