1kps: Difference between revisions

Revision as of 23:01, 2 May 2008

Structural Basis for E2-mediated SUMO conjugation revealed by a complex between ubiquitin conjugating enzyme Ubc9 and RanGAP1

OverviewOverview

E2 enzymes catalyze attachment of ubiquitin and ubiquitin-like proteins to lysine residues directly or through E3-mediated reactions. The small ubiquitin-like modifier SUMO regulates nuclear transport, stress response, and signal transduction in eukaryotes and is essential for cell-cycle progression in yeast. In contrast to most ubiquitin conjugation, the SUMO E2 enzyme Ubc9 is sufficient for substrate recognition and lysine modification of known SUMO targets. Crystallographic analysis of a complex between mammalian Ubc9 and a C-terminal domain of RanGAP1 at 2.5 A reveals structural determinants for recognition of consensus SUMO modification sequences found within SUMO-conjugated proteins. Structure-based mutagenesis and biochemical analysis of Ubc9 and RanGAP1 reveal distinct motifs required for substrate binding and SUMO modification of p53, IkappaBalpha, and RanGAP1.

About this StructureAbout this Structure

1KPS is a Protein complex structure of sequences from Homo sapiens and Mus musculus. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis for E2-mediated SUMO conjugation revealed by a complex between ubiquitin-conjugating enzyme Ubc9 and RanGAP1., Bernier-Villamor V, Sampson DA, Matunis MJ, Lima CD, Cell. 2002 Feb 8;108(3):345-56. PMID:11853669 Page seeded by OCA on Fri May 2 23:01:29 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1kps.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1kps |SIZE=350|CAPTION= <scene name='initialview01'>1kps</scene>, resolution 2.50&Aring;
+The line below this paragraph, containing "STRUCTURE_1kps", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= UBC9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), RANGAP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])
+-->
-|DOMAIN=
+{{STRUCTURE_1kps|  PDB=1kps  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kps FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kps OCA], [http://www.ebi.ac.uk/pdbsum/1kps PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1kps RCSB]</span>
-}}
 '''Structural Basis for E2-mediated SUMO conjugation revealed by a complex between ubiquitin conjugating enzyme Ubc9 and RanGAP1'''
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 [[Category: Matunis, M J.]]
 [[Category: Sampson, D A.]]
-[[Category: conjugating enzyme]]
+[[Category: Conjugating enzyme]]
-[[Category: e2]]
+[[Category: E2]]
-[[Category: ligase]]
+[[Category: Ligase]]
-[[Category: small ubiquitin-like modifier]]
+[[Category: Small ubiquitin-like modifier]]
-[[Category: sumo]]
+[[Category: Sumo]]
-[[Category: thioester]]
+[[Category: Thioester]]
-[[Category: ubiquitin]]
+[[Category: Ubiquitin]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 23:01:29 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:51:51 2008''