1kma: Difference between revisions
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'''NMR Structure of the Domain-I of the Kazal-type Thrombin Inhibitor Dipetalin''' | '''NMR Structure of the Domain-I of the Kazal-type Thrombin Inhibitor Dipetalin''' | ||
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[[Category: Schlott, B.]] | [[Category: Schlott, B.]] | ||
[[Category: Wohnert, J.]] | [[Category: Wohnert, J.]] | ||
[[Category: | [[Category: Disulphide-rich small alpha+beta fold]] | ||
[[Category: | [[Category: Kazal-type]] | ||
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Revision as of 22:54, 2 May 2008
NMR Structure of the Domain-I of the Kazal-type Thrombin Inhibitor Dipetalin
OverviewOverview
The interaction of domains of the Kazal-type inhibitor protein dipetalin with the serine proteinases thrombin and trypsin is studied. The functional studies of the recombinantly expressed domains (Dip-I+II, Dip-I and Dip-II) allow the dissection of the thrombin inhibitory properties and the identification of Dip-I as a key contributor to thrombin/dipetalin complex stability and its inhibitory potency. Furthermore, Dip-I, but not Dip-II, forms a complex with trypsin resulting in an inhibition of the trypsin activity directed towards protein substrates. The high resolution NMR structure of the Dip-I domain is determined using multi-dimensional heteronuclear NMR spectroscopy. Dip-I exhibits the canonical Kazal-type fold with a central alpha-helix and a short two-stranded antiparallel beta-sheet. Molecular regions essential for inhibitor complex formation with thrombin and trypsin are identified. A comparison with molecular complexes of other Kazal-type thrombin and trypsin inhibitors by molecular modeling shows that the N-terminal segment of Dip-I fulfills the structural prerequisites for inhibitory interactions with either proteinase and explains the capacity of this single Kazal-type domain to interact with different proteinases.
About this StructureAbout this Structure
1KMA is a Single protein structure of sequence from Dipetalogaster maximus. Full crystallographic information is available from OCA.
ReferenceReference
Interaction of Kazal-type inhibitor domains with serine proteinases: biochemical and structural studies., Schlott B, Wohnert J, Icke C, Hartmann M, Ramachandran R, Guhrs KH, Glusa E, Flemming J, Gorlach M, Grosse F, Ohlenschlager O, J Mol Biol. 2002 Apr 26;318(2):533-46. PMID:12051857 Page seeded by OCA on Fri May 2 22:54:35 2008