Sandbox Reserved 1790: Difference between revisions
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==PP1C and MRAS== | ==PP1C and MRAS== | ||
The interactions between <scene name='95/952718/Mras_and_pp1c/1'>PP1C and MRAS</scene> are respectively mediated by four main <scene name='95/952716/Mras_and_pp1c/3'>interactions</scene>: ionic interactions are between D48-R188 and H53-D197, hydrogen bonds are between K36-Q198 and Q35-M190. The relative order of complex ordering is still an area of debate. Some experiments indicate that PP1C must bind to SHOC2 before MRAS binds but others indicated that PP1C and MRAS can bind to SHOC2 at the same time <ref name=" | The interactions between <scene name='95/952718/Mras_and_pp1c/1'>PP1C and MRAS</scene> are respectively mediated by four main <scene name='95/952716/Mras_and_pp1c/3'>interactions</scene>: ionic interactions are between D48-R188 and H53-D197, hydrogen bonds are between K36-Q198 and Q35-M190. As the complex forms, the active site for the dephosphorylation of RAF's S259 is oriented such that it remains accessible for RAF <ref name="Hauseman" />. The relative order of complex ordering is still an area of debate. Some experiments indicate that PP1C must bind to SHOC2 before MRAS binds<ref name="Lavoie" /> but others indicated that PP1C and MRAS can bind to SHOC2 at the same time <ref name="Hauseman" />. | ||
=Signaling Pathway= | =Signaling Pathway= | ||
The SMP signaling pathway begins with the formation of the SMP complex. Initially, a ligand must bind to a receptor tyrosine kinase. This signals SHOC2 to bind to PP1C forming a binary complex that then binds to the membrane bound MRAS. Some literature indicates that the three proteins bind at the same time but the order is largely unknown. Figure 2 shows the proteins binding one at a time. Once the SMP complex forms, its target is the NTpS also known as S259. The serine is directly dephosphorylated by PP1C by SHOC2 and MRAS increase its specificity for S259. | The SMP signaling pathway begins with the formation of the SMP complex. Initially, a ligand must bind to a receptor tyrosine kinase. This signals SHOC2 to bind to PP1C forming a binary complex that then binds to the membrane bound MRAS. Some literature indicates that the three proteins bind at the same time but the order is largely unknown. Figure 2 shows the proteins binding one at a time. Once the SMP complex forms, its target is the NTpS also known as S259. The serine is directly dephosphorylated by PP1C by SHOC2 and MRAS increase its specificity for S259. | ||