1kl9: Difference between revisions

Revision as of 22:52, 2 May 2008

Crystal structure of the N-terminal segment of Human eukaryotic initiation factor 2alpha

OverviewOverview

Eukaryotic translation initiation factor 2alpha (eIF2alpha) is a member of the eIF2 heterotrimeric complex that binds and delivers Met-tRNA(i)(Met) to the 40 S ribosomal subunit in a GTP-dependent manner. Phosphorylation/dephosphorylation of eIF2alpha at Ser-51 is the major regulator of protein synthesis in eukaryotic cells. Here, we report the first structural analysis on eIF2, the three-dimensional structure of a 22-kDa N-terminal portion of human eIF2alpha by x-ray diffraction at 1.9 A resolution. This structure contains two major domains. The N terminus is a beta-barrel with five antiparallel beta-strands in an oligonucleotide binding domain (OB domain) fold. The phosphorylation site (Ser-51) is on the loop connecting beta3 and beta4 in the OB domain. A helical domain follows the OB domain, and the first helix has extensive interactions, including a disulfide bridge, to fix its orientation with respect to the OB domain. The two domains meet along a negatively charged groove with highly conserved residues, indicating a likely site for protein-protein interaction.

About this StructureAbout this Structure

1KL9 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of the N-terminal segment of human eukaryotic translation initiation factor 2alpha., Nonato MC, Widom J, Clardy J, J Biol Chem. 2002 May 10;277(19):17057-61. Epub 2002 Feb 21. PMID:11859078 Page seeded by OCA on Fri May 2 22:52:31 2008

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OCA

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 '''Crystal structure of the N-terminal segment of Human eukaryotic initiation factor 2alpha'''
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-[[Category: helical domain]]
+[[Category: Helical domain]]
-[[Category: ob fold]]
+[[Category: Ob fold]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 22:52:31 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:50:07 2008''