8bis: Difference between revisions
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==Crystal structure of cystathionine gamma-lyase from Toxoplasma gondii in complex with DL-propargylglycine== | |||
<StructureSection load='8bis' size='340' side='right'caption='[[8bis]], [[Resolution|resolution]] 2.27Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8bis]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Toxoplasma_gondii Toxoplasma gondii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8BIS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8BIS FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2AG:(2S)-2-AMINOPENT-4-ENOIC+ACID'>2AG</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8bis FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8bis OCA], [https://pdbe.org/8bis PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8bis RCSB], [https://www.ebi.ac.uk/pdbsum/8bis PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8bis ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/B6K8Y1_TOXGV B6K8Y1_TOXGV] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Cystathionine gamma-lyase (CGL) is a PLP-dependent enzyme that catalyzes the last step of the reverse transsulfuration route for endogenous cysteine biosynthesis. The canonical CGL-catalyzed process consists of an alpha,gamma-elimination reaction that breaks down cystathionine into cysteine, alpha-ketobutyrate, and ammonia. In some species, the enzyme can alternatively use cysteine as a substrate, resulting in the production of hydrogen sulfide (H(2) S). Importantly, inhibition of the enzyme and consequently of its H(2) S production activity, makes multiresistant bacteria considerably more susceptible to antibiotics. Other organisms, such as Toxoplasma gondii, the causative agent of toxoplasmosis, encode a CGL enzyme (TgCGL) that almost exclusively catalyzes the canonical process, with only minor reactivity to cysteine. Interestingly, the substitution of N360 by a serine (the equivalent amino acid residue in the human enzyme) at the active site changes the specificity of TgCGL for the catalysis of cystathionine, resulting in an enzyme that can cleave both the CgammaS and the CbetaS bond of cystathionine. Based on these findings and to deepen the molecular basis underlying the enzyme-substrate specificity, we have elucidated the crystal structures of native TgCGL and the variant TgCGL-N360S from crystals grown in the presence of cystathionine, cysteine, and the inhibitor d,l-propargylglycine (PPG). Our structures reveal the binding mode of each molecule within the catalytic cavity and help explain the inhibitory behavior of cysteine and PPG. A specific inhibitory mechanism of TgCGL by PPG is proposed. | |||
Structural basis of the inhibition of cystathionine gamma-lyase from Toxoplasma gondii by propargylglycine and cysteine.,Fernandez-Rodriguez C, Conter C, Oyenarte I, Favretto F, Quintana I, Martinez-Chantar ML, Astegno A, Martinez-Cruz LA Protein Sci. 2023 Apr;32(4):e4619. doi: 10.1002/pro.4619. PMID:36883335<ref>PMID:36883335</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 8bis" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Toxoplasma gondii]] | |||
[[Category: Astegno A]] | |||
[[Category: Conter C]] | |||
[[Category: Favretto F]] | |||
[[Category: Fernandez-Rodriguez C]] | |||
[[Category: Martinez-Chantar ML]] | |||
[[Category: Martinez-Cruz LA]] | |||
[[Category: Oyenarte I]] | |||
[[Category: Quintana I]] | |||
Revision as of 23:30, 12 April 2023
Crystal structure of cystathionine gamma-lyase from Toxoplasma gondii in complex with DL-propargylglycineCrystal structure of cystathionine gamma-lyase from Toxoplasma gondii in complex with DL-propargylglycine
Structural highlights
FunctionPublication Abstract from PubMedCystathionine gamma-lyase (CGL) is a PLP-dependent enzyme that catalyzes the last step of the reverse transsulfuration route for endogenous cysteine biosynthesis. The canonical CGL-catalyzed process consists of an alpha,gamma-elimination reaction that breaks down cystathionine into cysteine, alpha-ketobutyrate, and ammonia. In some species, the enzyme can alternatively use cysteine as a substrate, resulting in the production of hydrogen sulfide (H(2) S). Importantly, inhibition of the enzyme and consequently of its H(2) S production activity, makes multiresistant bacteria considerably more susceptible to antibiotics. Other organisms, such as Toxoplasma gondii, the causative agent of toxoplasmosis, encode a CGL enzyme (TgCGL) that almost exclusively catalyzes the canonical process, with only minor reactivity to cysteine. Interestingly, the substitution of N360 by a serine (the equivalent amino acid residue in the human enzyme) at the active site changes the specificity of TgCGL for the catalysis of cystathionine, resulting in an enzyme that can cleave both the CgammaS and the CbetaS bond of cystathionine. Based on these findings and to deepen the molecular basis underlying the enzyme-substrate specificity, we have elucidated the crystal structures of native TgCGL and the variant TgCGL-N360S from crystals grown in the presence of cystathionine, cysteine, and the inhibitor d,l-propargylglycine (PPG). Our structures reveal the binding mode of each molecule within the catalytic cavity and help explain the inhibitory behavior of cysteine and PPG. A specific inhibitory mechanism of TgCGL by PPG is proposed. Structural basis of the inhibition of cystathionine gamma-lyase from Toxoplasma gondii by propargylglycine and cysteine.,Fernandez-Rodriguez C, Conter C, Oyenarte I, Favretto F, Quintana I, Martinez-Chantar ML, Astegno A, Martinez-Cruz LA Protein Sci. 2023 Apr;32(4):e4619. doi: 10.1002/pro.4619. PMID:36883335[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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