Sandbox Reserved 1788: Difference between revisions
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== SHOC2 and MRAS == | == SHOC2 and MRAS == | ||
MRAS is initially bound to GDP causing it to be in its inactive state. This form cannot bind to the SHOC2-PP1C complex due to steric clashing. Once GDP is exchanged for GTP to activate the protein, <scene name='95/952716/conformational changes/1'>SHOC2-MRAS (residues)</scene> occur within the switch I and switch II regions to allow <scene name='95/952716/MRAS to interact with SHOC2/2'>SHOC2-MRAS(full-image)</scene>. These <scene name='95/952716/Scho2-mras-interactions/1'>interactions</scene> include hydrogen bonds and pi stacking. The primary hydrogen bonds are R288-Q71 and R177-E47. Pi staking occurs at R104-R83. | MRAS is initially bound to GDP causing it to be in its inactive state. This form cannot bind to the SHOC2-PP1C complex due to steric clashing (Figure 2). Once GDP is exchanged for GTP to activate the protein, <scene name='95/952716/conformational changes/1'>SHOC2-MRAS (residues)</scene> occur within the switch I and switch II regions to allow <scene name='95/952716/MRAS to interact with SHOC2/2'>SHOC2-MRAS(full-image)</scene>. These <scene name='95/952716/Scho2-mras-interactions/1'>interactions</scene> include hydrogen bonds and pi stacking. The primary hydrogen bonds are R288-Q71 and R177-E47. Pi staking occurs at R104-R83. | ||
[[Image:Switches.png|500 px|right| thumb|'''Figure 2:'''Steric clashing of Switch I and II of GDP bound MRAS, in green, with the surface of SHOC2, in magenta. GTP-bound MRAS, in white, shows no steric clashing with SHOC2s surface.]] | [[Image:Switches.png|500 px|right| thumb|'''Figure 2:'''Steric clashing of Switch I and II of GDP bound MRAS, in green, with the surface of SHOC2, in magenta. GTP-bound MRAS, in white, shows no steric clashing with SHOC2s surface.]] | ||