4w6x: Difference between revisions
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==Co-complex structure of the lectin domain of F18 fimbrial adhesin FedF with inhibitory nanobody NbFedF7== | ==Co-complex structure of the lectin domain of F18 fimbrial adhesin FedF with inhibitory nanobody NbFedF7== | ||
<StructureSection load='4w6x' size='340' side='right' caption='[[4w6x]], [[Resolution|resolution]] 1.88Å' scene=''> | <StructureSection load='4w6x' size='340' side='right'caption='[[4w6x]], [[Resolution|resolution]] 1.88Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4w6x]] is a 2 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4w6x]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W6X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4W6X FirstGlance]. <br> | ||
</td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4w6x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w6x OCA], [https://pdbe.org/4w6x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4w6x RCSB], [https://www.ebi.ac.uk/pdbsum/4w6x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4w6x ProSAT]</span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/Q47212_ECOLX Q47212_ECOLX] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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==See Also== | ==See Also== | ||
*[[Adhesin|Adhesin]] | *[[Adhesin 3D structures|Adhesin 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Escherichia coli]] | ||
[[Category: | [[Category: Lama glama]] | ||
[[Category: Coddens | [[Category: Large Structures]] | ||
[[Category: Cox | [[Category: Coddens A]] | ||
[[Category: Greve | [[Category: Cox E]] | ||
[[Category: Kerpel | [[Category: De Greve H]] | ||
[[Category: Moonens | [[Category: De Kerpel M]] | ||
[[Category: Pardon | [[Category: Moonens K]] | ||
[[Category: Remaut | [[Category: Pardon E]] | ||
[[Category: Remaut H]] | |||
Revision as of 09:55, 7 April 2023
Co-complex structure of the lectin domain of F18 fimbrial adhesin FedF with inhibitory nanobody NbFedF7Co-complex structure of the lectin domain of F18 fimbrial adhesin FedF with inhibitory nanobody NbFedF7
Structural highlights
FunctionPublication Abstract from PubMedPost-weaning diarrhea and edema disease caused by F18 fimbriated E. coli are important diseases in newly weaned piglets and lead to severe production losses in farming industry. Protective treatments against these infections have thus far limited efficacy. In this study we generated nanobodies directed against the lectin domain of the F18 fimbrial adhesin FedF and showed in an in vitro adherence assay that four unique nanobodies inhibit the attachment of F18 fimbriated E. coli bacteria to piglet enterocytes. Crystallization of the FedF lectin domain with the most potent inhibitory nanobodies revealed their mechanism of action. These either competed with the binding of the blood group antigen receptor on the FedF surface or induced a conformational change in which the CDR3 region of the nanobody displaces the D-E loop adjacent to the binding site. This D-E loop was previously shown to be required for the interaction between F18 fimbriated bacteria and blood group antigen receptors in a membrane context. This work demonstrates the feasibility of inhibiting the attachment of fimbriated pathogens by employing nanobodies directed against the adhesin domain. Nanobody Mediated Inhibition of Attachment of F18 Fimbriae Expressing Escherichia coli.,Moonens K, De Kerpel M, Coddens A, Cox E, Pardon E, Remaut H, De Greve H PLoS One. 2014 Dec 11;9(12):e114691. doi: 10.1371/journal.pone.0114691., eCollection 2014. PMID:25502211[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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