1kc8: Difference between revisions

Revision as of 22:34, 2 May 2008

Co-crystal Structure of Blasticidin S Bound to the 50S Ribosomal Subunit

OverviewOverview

Structures of anisomycin, chloramphenicol, sparsomycin, blasticidin S, and virginiamycin M bound to the large ribosomal subunit of Haloarcula marismortui have been determined at 3.0A resolution. Most of these antibiotics bind to sites that overlap those of either peptidyl-tRNA or aminoacyl-tRNA, consistent with their functioning as competitive inhibitors of peptide bond formation. Two hydrophobic crevices, one at the peptidyl transferase center and the other at the entrance to the peptide exit tunnel play roles in binding these antibiotics. Midway between these crevices, nucleotide A2103 of H.marismortui (2062 Escherichia coli) varies in its conformation and thereby contacts antibiotics bound at either crevice. The aromatic ring of anisomycin binds to the active-site hydrophobic crevice, as does the aromatic ring of puromycin, while the aromatic ring of chloramphenicol binds to the exit tunnel hydrophobic crevice. Sparsomycin contacts primarily a P-site bound substrate, but also extends into the active-site hydrophobic crevice. Virginiamycin M occupies portions of both the A and P-site, and induces a conformational change in the ribosome. Blasticidin S base-pairs with the P-loop and thereby mimics C74 and C75 of a P-site bound tRNA.

About this StructureAbout this Structure

1KC8 is a Protein complex structure of sequences from Haloarcula marismortui. Full crystallographic information is available from OCA.

ReferenceReference

Structures of five antibiotics bound at the peptidyl transferase center of the large ribosomal subunit., Hansen JL, Moore PB, Steitz TA, J Mol Biol. 2003 Jul 25;330(5):1061-75. PMID:12860128 Page seeded by OCA on Fri May 2 22:34:04 2008

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OCA

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 [[Image:1kc8.gif|left|200px]]
- {{Structure
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-|RELATEDENTRY=[[1k73|1K73]], [[1k8a|1K8A]], [[1k9m|1K9M]], [[1kd1|1KD1]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kc8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kc8 OCA], [http://www.ebi.ac.uk/pdbsum/1kc8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1kc8 RCSB]</span>
-}}
 '''Co-crystal Structure of Blasticidin S Bound to the 50S Ribosomal Subunit'''
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 [[Category: Steitz, T A.]]
 [[Category: 50s subunit]]
-[[Category: antibiotic]]
+[[Category: Antibiotic]]
-[[Category: blasticidin s]]
+[[Category: Blasticidin s]]
-[[Category: p-loop]]
+[[Category: P-loop]]
-[[Category: p-site]]
+[[Category: P-site]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 22:34:04 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:46:32 2008''