7ygc: Difference between revisions
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==Cryo-EM structure of Tetrahymena ribozyme conformation 4 undergoing the second-step self-splicing== | |||
<StructureSection load='7ygc' size='340' side='right'caption='[[7ygc]], [[Resolution|resolution]] 2.65Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7ygc]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Tetrahymena Tetrahymena]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YGC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YGC FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SSU:URIDINE-5-PHOSPHOROTHIOATE'>SSU</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ygc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ygc OCA], [https://pdbe.org/7ygc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ygc RCSB], [https://www.ebi.ac.uk/pdbsum/7ygc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ygc ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Group I introns are catalytic RNAs that coordinate two consecutive transesterification reactions for self-splicing. To understand how the group I intron promotes catalysis and coordinates self-splicing reactions, we determine the structures of L-16 Tetrahymena ribozyme in complex with a 5'-splice site analog product and a 3'-splice site analog substrate using cryo-EM. We solve six conformations from a single specimen, corresponding to different splicing intermediates after the first ester-transfer reaction. The structures reveal dynamics during self-splicing, including large conformational changes of the internal guide sequence and the J5/4 junction as well as subtle rearrangements of active-site metals and the hydrogen bond formed between the 2'-OH group of A261 and the N2 group of guanosine substrate. These results help complete a detailed structural and mechanistic view of this paradigmatic group I intron undergoing the second step of self-splicing. | |||
Snapshots of the second-step self-splicing of Tetrahymena ribozyme revealed by cryo-EM.,Li S, Palo MZ, Zhang X, Pintilie G, Zhang K Nat Commun. 2023 Mar 16;14(1):1294. doi: 10.1038/s41467-023-36724-5. PMID:36928031<ref>PMID:36928031</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 7ygc" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Tetrahymena]] | |||
[[Category: Greg P]] | |||
[[Category: Li S]] | |||
[[Category: Michael ZP]] | |||
[[Category: Zhang K]] | |||
[[Category: Zhang X]] | |||
Revision as of 09:44, 29 March 2023
Cryo-EM structure of Tetrahymena ribozyme conformation 4 undergoing the second-step self-splicingCryo-EM structure of Tetrahymena ribozyme conformation 4 undergoing the second-step self-splicing
Structural highlights
Publication Abstract from PubMedGroup I introns are catalytic RNAs that coordinate two consecutive transesterification reactions for self-splicing. To understand how the group I intron promotes catalysis and coordinates self-splicing reactions, we determine the structures of L-16 Tetrahymena ribozyme in complex with a 5'-splice site analog product and a 3'-splice site analog substrate using cryo-EM. We solve six conformations from a single specimen, corresponding to different splicing intermediates after the first ester-transfer reaction. The structures reveal dynamics during self-splicing, including large conformational changes of the internal guide sequence and the J5/4 junction as well as subtle rearrangements of active-site metals and the hydrogen bond formed between the 2'-OH group of A261 and the N2 group of guanosine substrate. These results help complete a detailed structural and mechanistic view of this paradigmatic group I intron undergoing the second step of self-splicing. Snapshots of the second-step self-splicing of Tetrahymena ribozyme revealed by cryo-EM.,Li S, Palo MZ, Zhang X, Pintilie G, Zhang K Nat Commun. 2023 Mar 16;14(1):1294. doi: 10.1038/s41467-023-36724-5. PMID:36928031[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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