1kbh: Difference between revisions

Revision as of 22:32, 2 May 2008

Mutual Synergistic Folding in the Interaction Between Nuclear Receptor Coactivators CBP and ACTR

OverviewOverview

Nuclear hormone receptors are ligand-activated transcription factors that regulate the expression of genes that are essential for development, reproduction and homeostasis. The hormone response is mediated through recruitment of p160 receptor coactivators and the general transcriptional coactivator CBP/p300, which function synergistically to activate transcription. These coactivators exhibit intrinsic histone acetyltransferase activity, function in the remodelling of chromatin, and facilitate the recruitment of RNA polymerase II and the basal transcription machinery. The activities of the p160 coactivators are dependent on CBP. Both coactivators are essential for proper cell-cycle control, differentiation and apoptosis, and are implicated in cancer and other diseases. To elucidate the molecular basis of assembling the multiprotein activation complex, we undertook a structural and thermodynamic analysis of the interaction domains of CBP and the activator for thyroid hormone and retinoid receptors. Here we show that although the isolated domains are intrinsically disordered, they combine with high affinity to form a cooperatively folded helical heterodimer. Our study uncovers a unique mechanism, called 'synergistic folding', through which p160 coactivators recruit CBP/p300 to allow transmission of the hormonal signal to the transcriptional machinery.

About this StructureAbout this Structure

1KBH is a Protein complex structure of sequences from Homo sapiens and Mus musculus. Full crystallographic information is available from OCA.

ReferenceReference

Mutual synergistic folding in recruitment of CBP/p300 by p160 nuclear receptor coactivators., Demarest SJ, Martinez-Yamout M, Chung J, Chen H, Xu W, Dyson HJ, Evans RM, Wright PE, Nature. 2002 Jan 31;415(6871):549-53. PMID:11823864 Page seeded by OCA on Fri May 2 22:32:25 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1kbh.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1kbh |SIZE=350|CAPTION= <scene name='initialview01'>1kbh</scene>
+The line below this paragraph, containing "STRUCTURE_1kbh", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND=
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE= ACTR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), CBP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])
+-->
-|DOMAIN=
+{{STRUCTURE_1kbh|  PDB=1kbh  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kbh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kbh OCA], [http://www.ebi.ac.uk/pdbsum/1kbh PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1kbh RCSB]</span>
-}}
 '''Mutual Synergistic Folding in the Interaction Between Nuclear Receptor Coactivators CBP and ACTR'''
@@ Line 34: / Line 31: @@
 [[Category: Wright, P E.]]
 [[Category: Xu, W.]]
-[[Category: actr]]
+[[Category: Actr]]
-[[Category: cbp]]
+[[Category: Cbp]]
-[[Category: coactivator]]
+[[Category: Coactivator]]
-[[Category: creb-binding protein]]
+[[Category: Creb-binding protein]]
-[[Category: nuclear hormone receptor]]
+[[Category: Nuclear hormone receptor]]
-[[Category: p160]]
+[[Category: P160]]
-[[Category: p300]]
+[[Category: P300]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 22:32:25 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:46:08 2008''