2po4: Difference between revisions
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<StructureSection load='2po4' size='340' side='right'caption='[[2po4]], [[Resolution|resolution]] 2.00Å' scene=''> | <StructureSection load='2po4' size='340' side='right'caption='[[2po4]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2po4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[2po4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_N4 Escherichia virus N4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PO4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PO4 FirstGlance]. <br> | ||
</td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2po4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2po4 OCA], [https://pdbe.org/2po4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2po4 RCSB], [https://www.ebi.ac.uk/pdbsum/2po4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2po4 ProSAT]</span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2po4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2po4 OCA], [https://pdbe.org/2po4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2po4 RCSB], [https://www.ebi.ac.uk/pdbsum/2po4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2po4 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/RPOLV_BPN4 RPOLV_BPN4] DNA-dependent RNA polymerase, which is injected into the host upon infection and transcribes the phage early genes from promoters that have a 5-bp stem-3 nt loop hairpin structure.<ref>PMID:18362338</ref> <ref>PMID:19061645</ref> | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Escherichia virus N4]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Davydova | [[Category: Davydova EK]] | ||
[[Category: Murakami | [[Category: Murakami KS]] | ||
[[Category: Rothman-Denes | [[Category: Rothman-Denes LB]] | ||
Revision as of 10:46, 22 March 2023
X-ray crystal structure of polymerase domain of the bacteriophage N4 virion RNA polymeraseX-ray crystal structure of polymerase domain of the bacteriophage N4 virion RNA polymerase
Structural highlights
FunctionRPOLV_BPN4 DNA-dependent RNA polymerase, which is injected into the host upon infection and transcribes the phage early genes from promoters that have a 5-bp stem-3 nt loop hairpin structure.[1] [2] Publication Abstract from PubMedColiphage N4 virion RNA polymerase (vRNAP), which is injected into the host upon infection, transcribes the phage early genes from promoters that have a 5-bp stem-3 nt loop hairpin structure. Here, we describe the 2.0-A resolution x-ray crystal structure of N4 mini-vRNAP, a member of the T7-like, single-unit RNAP family and the minimal component having all RNAP functions of the full-length vRNAP. The structure resembles a "fisted right hand" with Fingers, Palm and Thumb subdomains connected to an N-terminal domain. We established that the specificity loop extending from the Fingers along with W129 of the N-terminal domain play critical roles in hairpin-promoter recognition. A comparison with the structure of the T7 RNAP initiation complex reveals that the pathway of the DNA to the active site is blocked in the apo-form vRNAP, indicating that vRNAP must undergo a large-scale conformational change upon promoter DNA binding and explaining the highly restricted promoter specificity of vRNAP that is essential for phage early transcription. X-ray crystal structure of the polymerase domain of the bacteriophage N4 virion RNA polymerase.,Murakami KS, Davydova EK, Rothman-Denes LB Proc Natl Acad Sci U S A. 2008 Mar 24;. PMID:18362338[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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