8hil: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==A cryo-EM structure of B. oleracea RNA polymerase V at 3.57 Angstrom== | |||
<StructureSection load='8hil' size='340' side='right'caption='[[8hil]], [[Resolution|resolution]] 3.57Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8hil]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Brassica_oleracea Brassica oleracea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8HIL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8HIL FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8hil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8hil OCA], [https://pdbe.org/8hil PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8hil RCSB], [https://www.ebi.ac.uk/pdbsum/8hil PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8hil ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A0D3D418_BRAOL A0A0D3D418_BRAOL] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
In addition to the conserved RNA polymerases (Pols) I-III in eukaryotes, two atypical polymerases, Pols IV and V, specifically produce non-coding RNA in the RNA-directed DNA methylation (RdDM) pathway in plants. Here, we report on the structures of cauliflower Pol V in the free and elongation conformations. A conserved tyrosine residue of NRPE2 stacks with a dsDNA branch of the transcription bubble to potentially attenuate elongation by inducing transcription stalling. The non-template DNA strand is captured by NRPE2 to enhance backtracking, thereby increasing 3'-5' cleavage which likely underpins Pol V's high fidelity. The structures also illuminate the mechanism of Pol V transcription stalling and enhanced backtracking which may be important for Pol V's retention on chromatin to serve its function in tethering downstream factors for RdDM. | |||
Structure and mechanism of the plant RNA polymerase V.,Xie G, Du X, Hu H, Li S, Cao X, Jacobsen SE, Du J Science. 2023 Mar 9:eadf8231. doi: 10.1126/science.adf8231. PMID:36893216<ref>PMID:36893216</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 8hil" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Brassica oleracea]] | |||
[[Category: Large Structures]] | |||
[[Category: Du J]] | |||
[[Category: Du X]] | |||
[[Category: Hu H]] | |||
[[Category: Xie G]] | |||
Revision as of 10:28, 22 March 2023
A cryo-EM structure of B. oleracea RNA polymerase V at 3.57 AngstromA cryo-EM structure of B. oleracea RNA polymerase V at 3.57 Angstrom
Structural highlights
FunctionPublication Abstract from PubMedIn addition to the conserved RNA polymerases (Pols) I-III in eukaryotes, two atypical polymerases, Pols IV and V, specifically produce non-coding RNA in the RNA-directed DNA methylation (RdDM) pathway in plants. Here, we report on the structures of cauliflower Pol V in the free and elongation conformations. A conserved tyrosine residue of NRPE2 stacks with a dsDNA branch of the transcription bubble to potentially attenuate elongation by inducing transcription stalling. The non-template DNA strand is captured by NRPE2 to enhance backtracking, thereby increasing 3'-5' cleavage which likely underpins Pol V's high fidelity. The structures also illuminate the mechanism of Pol V transcription stalling and enhanced backtracking which may be important for Pol V's retention on chromatin to serve its function in tethering downstream factors for RdDM. Structure and mechanism of the plant RNA polymerase V.,Xie G, Du X, Hu H, Li S, Cao X, Jacobsen SE, Du J Science. 2023 Mar 9:eadf8231. doi: 10.1126/science.adf8231. PMID:36893216[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
|
| ||||||||||||||||