2n8v: Difference between revisions
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==An NMR/SAXS structure of the PKI domain of the honeybee dicistrovirus, Israeli acute paralysis virus (IAPV) IRES== | ==An NMR/SAXS structure of the PKI domain of the honeybee dicistrovirus, Israeli acute paralysis virus (IAPV) IRES== | ||
<StructureSection load='2n8v' size='340' side='right'caption='[[2n8v | <StructureSection load='2n8v' size='340' side='right'caption='[[2n8v]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2n8v]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N8V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N8V FirstGlance]. <br> | <table><tr><td colspan='2'>[[2n8v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Apis_mellifera Apis mellifera]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N8V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N8V FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n8v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n8v OCA], [https://pdbe.org/2n8v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n8v RCSB], [https://www.ebi.ac.uk/pdbsum/2n8v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n8v ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n8v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n8v OCA], [https://pdbe.org/2n8v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n8v RCSB], [https://www.ebi.ac.uk/pdbsum/2n8v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n8v ProSAT]</span></td></tr> | ||
</table> | </table> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Apis mellifera]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Au | [[Category: Au HH]] | ||
[[Category: Burke | [[Category: Burke JE]] | ||
[[Category: Butcher | [[Category: Butcher SE]] | ||
[[Category: Cornilescu | [[Category: Cornilescu G]] | ||
[[Category: Jan | [[Category: Jan E]] | ||
[[Category: Lee | [[Category: Lee S]] | ||
[[Category: Mouzakis | [[Category: Mouzakis KD]] | ||
[[Category: Ren | [[Category: Ren Q]] | ||
Revision as of 13:23, 15 March 2023
An NMR/SAXS structure of the PKI domain of the honeybee dicistrovirus, Israeli acute paralysis virus (IAPV) IRESAn NMR/SAXS structure of the PKI domain of the honeybee dicistrovirus, Israeli acute paralysis virus (IAPV) IRES
Structural highlights
Publication Abstract from PubMedThe dicistrovirus intergenic region internal ribosome entry site (IRES) adopts a triple-pseudoknotted RNA structure and occupies the core ribosomal E, P, and A sites to directly recruit the ribosome and initiate translation at a non-AUG codon. A subset of dicistrovirus IRESs directs translation in the 0 and +1 frames to produce the viral structural proteins and a +1 overlapping open reading frame called ORFx, respectively. Here we show that specific mutations of two unpaired adenosines located at the core of the three-helical junction of the honey bee dicistrovirus Israeli acute paralysis virus (IAPV) IRES PKI domain can uncouple 0 and +1 frame translation, suggesting that the structure adopts distinct conformations that contribute to 0 or +1 frame translation. Using a reconstituted translation system, we show that ribosomes assembled on mutant IRESs that direct exclusive 0 or +1 frame translation lack reading frame fidelity. Finally, a nuclear magnetic resonance/small-angle X-ray scattering hybrid approach reveals that the PKI domain of the IAPV IRES adopts an RNA structure that resembles a complete tRNA. The tRNA shape-mimicry enables the viral IRES to gain access to the ribosome tRNA-binding sites and form intermolecular contacts with the ribosome that are necessary for initiating IRES translation in a specific reading frame. Global shape mimicry of tRNA within a viral internal ribosome entry site mediates translational reading frame selection.,Au HH, Cornilescu G, Mouzakis KD, Ren Q, Burke JE, Lee S, Butcher SE, Jan E Proc Natl Acad Sci U S A. 2015 Nov 24;112(47):E6446-55. doi:, 10.1073/pnas.1512088112. Epub 2015 Nov 9. PMID:26554019[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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