2n7h: Difference between revisions
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==Hybrid structure of the Type 1 Pilus of Uropathogenic E.coli== | ==Hybrid structure of the Type 1 Pilus of Uropathogenic E.coli== | ||
<StructureSection load='2n7h' size='340' side='right'caption='[[2n7h | <StructureSection load='2n7h' size='340' side='right'caption='[[2n7h]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2n7h]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[2n7h]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2mx3 2mx3]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N7H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N7H FirstGlance]. <br> | ||
</td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n7h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n7h OCA], [https://pdbe.org/2n7h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n7h RCSB], [https://www.ebi.ac.uk/pdbsum/2n7h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n7h ProSAT]</span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n7h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n7h OCA], [https://pdbe.org/2n7h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n7h RCSB], [https://www.ebi.ac.uk/pdbsum/2n7h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n7h ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/FIMA1_ECOLI FIMA1_ECOLI] Fimbriae (also called pili), polar filaments radiating from the surface of the bacterium to a length of 0.5-1.5 micrometers and numbering 100-300 per cell, enable bacteria to colonize the epithelium of specific host organs. | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Escherichia coli]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Becker | [[Category: Becker S]] | ||
[[Category: Giller | [[Category: Giller K]] | ||
[[Category: Habeck | [[Category: Habeck M]] | ||
[[Category: Habenstein | [[Category: Habenstein B]] | ||
[[Category: Lange | [[Category: Lange A]] | ||
[[Category: Loquet | [[Category: Loquet A]] | ||
[[Category: Vasa | [[Category: Vasa S]] | ||
Latest revision as of 13:22, 15 March 2023
Hybrid structure of the Type 1 Pilus of Uropathogenic E.coliHybrid structure of the Type 1 Pilus of Uropathogenic E.coli
Structural highlights
FunctionFIMA1_ECOLI Fimbriae (also called pili), polar filaments radiating from the surface of the bacterium to a length of 0.5-1.5 micrometers and numbering 100-300 per cell, enable bacteria to colonize the epithelium of specific host organs. Publication Abstract from PubMedType 1 pili are filamentous protein assemblies on the surface of Gram-negative bacteria that mediate adhesion to host cells during the infection process. The molecular structure of type 1 pili remains elusive on the atomic scale owing to their insolubility and noncrystallinity. Herein we describe an approach for hybrid-structure determination that is based on data from solution-state NMR spectroscopy on the soluble subunit and solid-state NMR spectroscopy and STEM data on the assembled pilus. Our approach is based on iterative modeling driven by structural information extracted from different sources and provides a general tool to access pseudo atomic structures of protein assemblies with complex subunit folds. By using this methodology, we determined the local conformation of the FimA pilus subunit in the context of the assembled type 1 pilus, determined the exact helical pilus architecture, and elucidated the intermolecular interfaces contributing to pilus assembly and stability with atomic detail. Hybrid Structure of the Type 1 Pilus of Uropathogenic Escherichia coli.,Habenstein B, Loquet A, Hwang S, Giller K, Vasa SK, Becker S, Habeck M, Lange A Angew Chem Int Ed Engl. 2015 Aug 12. doi: 10.1002/anie.201505065. PMID:26267365[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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