2n01: Difference between revisions
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==NMR structure of VirB9 C-terminal domain in complex with VirB7 N-terminal domain from Xanthomonas citri's T4SS== | ==NMR structure of VirB9 C-terminal domain in complex with VirB7 N-terminal domain from Xanthomonas citri's T4SS== | ||
<StructureSection load='2n01' size='340' side='right'caption='[[2n01 | <StructureSection load='2n01' size='340' side='right'caption='[[2n01]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2n01]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[2n01]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Xanthomonas_citri_pv._citri_str._306 Xanthomonas citri pv. citri str. 306]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N01 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N01 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n01 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n01 OCA], [https://pdbe.org/2n01 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n01 RCSB], [https://www.ebi.ac.uk/pdbsum/2n01 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n01 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n01 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n01 OCA], [https://pdbe.org/2n01 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n01 RCSB], [https://www.ebi.ac.uk/pdbsum/2n01 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n01 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/Q8PJB3_XANAC Q8PJB3_XANAC] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Xanthomonas citri pv. citri str. 306]] | ||
[[Category: Boelens | [[Category: Boelens R]] | ||
[[Category: Farah | [[Category: Farah SC]] | ||
[[Category: Oliveira | [[Category: Oliveira LC]] | ||
[[Category: Salinas | [[Category: Salinas RK]] | ||
[[Category: Souza | [[Category: Souza DP]] | ||
[[Category: Wienk | [[Category: Wienk H]] | ||
Revision as of 11:12, 8 March 2023
NMR structure of VirB9 C-terminal domain in complex with VirB7 N-terminal domain from Xanthomonas citri's T4SSNMR structure of VirB9 C-terminal domain in complex with VirB7 N-terminal domain from Xanthomonas citri's T4SS
Structural highlights
FunctionPublication Abstract from PubMedThe type IV secretion system (T4SS) from the phytopathogen Xanthomonas citri (Xac) is a bactericidal nanomachine. The T4SS core complex is a ring composed of multiple copies of VirB7-VirB9-VirB10 subunits. Xac-VirB7 contains a disordered N-terminal tail (VirB7NT) that recognizes VirB9, and a C-terminal domain (VirB7CT) involved in VirB7 self-association. Here, we show that VirB7NT forms a short beta strand upon binding to VirB9 and stabilizes it. A tight interaction between them is essential for T4SS assembly and antibacterial activity. Abolishing VirB7 self-association or deletion of the VirB7 C-terminal domain impairs this antibacterial activity without disturbing T4SS assembly. These findings reveal protein interactions within the core complex that are critical for the stability and activity of a T4SS. VirB7 and VirB9 Interactions Are Required for the Assembly and Antibacterial Activity of a Type IV Secretion System.,Oliveira LC, Souza DP, Oka GU, Lima FD, Oliveira RJ, Favaro DC, Wienk H, Boelens R, Farah CS, Salinas RK Structure. 2016 Oct 4;24(10):1707-1718. doi: 10.1016/j.str.2016.07.015. Epub 2016, Sep 1. PMID:27594685[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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