1k2w: Difference between revisions

Revision as of 22:14, 2 May 2008

Crystal structure of sorbitol dehydrogenase from R. sphaeroides

OverviewOverview

Recombinant sorbitol dehydrogenase (SDH) from Rhodobacter sphaeroides has been crystallized in the absence of the cofactor NAD(H) and its structure determined to 2.4 A resolution using molecular replacement (refined R and R free factors of 18.8 and 23.8%, respectively). As expected from the sequence and shown by the conserved fold, SDH can be assigned to the short-chain dehydrogenase/reductase protein family. The cofactor NAD and the substrate sorbitol have been modelled into the structure and the active-site architecture, which displays the highly conserved catalytic tetrad of Asn-Ser-Tyr-Lys residues, is discussed in relation to the enzyme mechanism. This is the first structure of a bacterial SDH belonging to the SDR family.

About this StructureAbout this Structure

1K2W is a Single protein structure of sequence from Rhodobacter sphaeroides. Full crystallographic information is available from OCA.

ReferenceReference

Structure of zinc-independent sorbitol dehydrogenase from Rhodobacter sphaeroides at 2.4 A resolution., Philippsen A, Schirmer T, Stein MA, Giffhorn F, Stetefeld J, Acta Crystallogr D Biol Crystallogr. 2005 Apr;61(Pt 4):374-9. Epub 2005, Mar 24. PMID:15805591 Page seeded by OCA on Fri May 2 22:14:12 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1k2w.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1k2w |SIZE=350|CAPTION= <scene name='initialview01'>1k2w</scene>, resolution 2.4&Aring;
+The line below this paragraph, containing "STRUCTURE_1k2w", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND=
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/L-iditol_2-dehydrogenase L-iditol 2-dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.14 1.1.1.14] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1k2w|  PDB=1k2w  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k2w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k2w OCA], [http://www.ebi.ac.uk/pdbsum/1k2w PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1k2w RCSB]</span>
-}}
 '''Crystal structure of sorbitol dehydrogenase from R. sphaeroides'''
@@ Line 29: / Line 26: @@
 [[Category: Schirmer, T.]]
 [[Category: Stetefeld, J.]]
-[[Category: enzyme]]
+[[Category: Enzyme]]
-[[Category: short-chain dehydrogenase]]
+[[Category: Short-chain dehydrogenase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 22:14:12 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:42:39 2008''