1k07: Difference between revisions

Revision as of 22:08, 2 May 2008

Native FEZ-1 metallo-beta-lactamase from Legionella gormanii

OverviewOverview

The beta-lactamases are involved in bacterial resistance to penicillin and related compounds. Members of the metallo-enzyme class are now found in many pathogenic bacteria and are thus becoming of major clinical importance. The structures of the Zn-beta-lactamase from Fluoribacter gormanii (FEZ-1) in the native and in the complex form are reported here. FEZ-1 is a monomeric enzyme, which possesses two zinc-binding sites. These structures are discussed in comparison with those of the tetrameric L1 enzyme produced by Stenotrophomonas maltophilia. From this analysis, amino acids involved in the oligomerization of L1 are clearly identified. Despite the similarity in fold, the active site of FEZ-1 was found to be significantly different. Two residues, which were previously implicated in function, are not present in L1 or in FEZ-1. The broad-spectrum substrate profile of Zn-beta-lactamases arises from the rather wide active-site cleft, where various beta-lactam compounds can be accommodated.

About this StructureAbout this Structure

1K07 is a Single protein structure of sequence from Fluoribacter gormanii. Full crystallographic information is available from OCA.

ReferenceReference

Three-dimensional structure of FEZ-1, a monomeric subclass B3 metallo-beta-lactamase from Fluoribacter gormanii, in native form and in complex with D-captopril., Garcia-Saez I, Mercuri PS, Papamicael C, Kahn R, Frere JM, Galleni M, Rossolini GM, Dideberg O, J Mol Biol. 2003 Jan 24;325(4):651-60. PMID:12507470 Page seeded by OCA on Fri May 2 22:08:12 2008

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OCA

@@ Line 1: / Line 1: @@
 [[Image:1k07.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1k07 |SIZE=350|CAPTION= <scene name='initialview01'>1k07</scene>, resolution 1.65&Aring;
+The line below this paragraph, containing "STRUCTURE_1k07", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= blaFEZ-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=464 Fluoribacter gormanii])
+-->
-|DOMAIN=
+{{STRUCTURE_1k07|  PDB=1k07  |  SCENE=  }}
-|RELATEDENTRY=[[1jt1|1JT1]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k07 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k07 OCA], [http://www.ebi.ac.uk/pdbsum/1k07 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1k07 RCSB]</span>
-}}
 '''Native FEZ-1 metallo-beta-lactamase from Legionella gormanii'''
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 [[Category: Mercuri, P S.]]
 [[Category: Papamicael, C.]]
-[[Category: monomer with alpha-beta/beta-alpha fold. two monomers per assymmetric unit.]]
+[[Category: Monomer with alpha-beta/beta-alpha fold. two monomers per assymmetric unit.]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 22:08:12 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:41:29 2008''