2m3g: Difference between revisions
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==Structure of Anabaena Sensory Rhodopsin Determined by Solid State NMR Spectroscopy== | ==Structure of Anabaena Sensory Rhodopsin Determined by Solid State NMR Spectroscopy== | ||
<StructureSection load='2m3g' size='340' side='right'caption='[[2m3g | <StructureSection load='2m3g' size='340' side='right'caption='[[2m3g]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2m3g]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[2m3g]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Nostoc_sp._PCC_7120_=_FACHB-418 Nostoc sp. PCC 7120 = FACHB-418]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M3G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2M3G FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2m3g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m3g OCA], [https://pdbe.org/2m3g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2m3g RCSB], [https://www.ebi.ac.uk/pdbsum/2m3g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2m3g ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2m3g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m3g OCA], [https://pdbe.org/2m3g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2m3g RCSB], [https://www.ebi.ac.uk/pdbsum/2m3g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2m3g ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/Q8YSC4_NOSS1 Q8YSC4_NOSS1] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Brown | [[Category: Nostoc sp. PCC 7120 = FACHB-418]] | ||
[[Category: Jung | [[Category: Brown LS]] | ||
[[Category: Kawamura | [[Category: Jung K]] | ||
[[Category: Kim | [[Category: Kawamura I]] | ||
[[Category: Ladizhansky | [[Category: Kim S]] | ||
[[Category: Munro | [[Category: Ladizhansky V]] | ||
[[Category: Okitsu | [[Category: Munro RA]] | ||
[[Category: Shi | [[Category: Okitsu T]] | ||
[[Category: Wada | [[Category: Shi L]] | ||
[[Category: Wang | [[Category: Wada A]] | ||
[[Category: Wang S]] | |||
Revision as of 16:27, 22 February 2023
Structure of Anabaena Sensory Rhodopsin Determined by Solid State NMR SpectroscopyStructure of Anabaena Sensory Rhodopsin Determined by Solid State NMR Spectroscopy
Structural highlights
FunctionPublication Abstract from PubMedDetermination of structure of integral membrane proteins, especially in their native environment, is a formidable challenge in structural biology. Here we demonstrate that magic angle spinning solid-state NMR spectroscopy can be used to determine structures of membrane proteins reconstituted in synthetic lipids, an environment similar to the natural membrane. We combined a large number of experimentally determined interatomic distances and local torsional restraints to solve the structure of an oligomeric membrane protein of common seven-helical fold, Anabaena sensory rhodopsin (ASR). We determined the atomic resolution detail of the oligomerization interface of the ASR trimer, and the arrangement of helices, side chains and the retinal cofactor in the monomer. Solid-state NMR spectroscopy structure determination of a lipid-embedded heptahelical membrane protein.,Wang S, Munro RA, Shi L, Kawamura I, Okitsu T, Wada A, Kim SY, Jung KH, Brown LS, Ladizhansky V Nat Methods. 2013 Sep 8. doi: 10.1038/nmeth.2635. PMID:24013819[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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