1jwe: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1jwe.gif|left|200px]] | [[Image:1jwe.gif|left|200px]] | ||
<!-- | |||
The line below this paragraph, containing "STRUCTURE_1jwe", creates the "Structure Box" on the page. | |||
You may change the PDB parameter (which sets the PDB file loaded into the applet) | |||
or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |||
| | or leave the SCENE parameter empty for the default display. | ||
| | --> | ||
{{STRUCTURE_1jwe| PDB=1jwe | SCENE= }} | |||
}} | |||
'''NMR Structure of the N-Terminal Domain of E. Coli Dnab Helicase''' | '''NMR Structure of the N-Terminal Domain of E. Coli Dnab Helicase''' | ||
| Line 30: | Line 27: | ||
[[Category: Otting, G.]] | [[Category: Otting, G.]] | ||
[[Category: Weigelt, J.]] | [[Category: Weigelt, J.]] | ||
[[Category: | [[Category: Helicase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:00:27 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 22:00, 2 May 2008
NMR Structure of the N-Terminal Domain of E. Coli Dnab Helicase
OverviewOverview
BACKGROUND: DnaB is the primary replicative helicase in Escherichia coli. Native DnaB is a hexamer of identical subunits, each consisting of a larger C-terminal domain and a smaller N-terminal domain. Electron-microscopy data show hexamers with C6 or C3 symmetry, indicating large domain movements and reversible pairwise association. RESULTS: The three-dimensional structure of the N-terminal domain of E. coli DnaB was determined by nuclear magnetic resonance (NMR) spectroscopy. Structural similarity was found with the primary dimerisation domain of a topoisomerase, the gyrase A subunit from E. coli. A monomer-dimer equilibrium was observed for the isolated N-terminal domain of DnaB. A dimer model with C2 symmetry was derived from intermolecular nuclear Overhauser effects, which is consistent with all available NMR data. CONCLUSIONS: The monomer-dimer equilibrium observed for the N-terminal domain of DnaB is likely to be of functional significance for helicase activity, by participating in the switch between C6 and C3 symmetry of the helicase hexamer.
About this StructureAbout this Structure
1JWE is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
NMR structure of the N-terminal domain of E. coli DnaB helicase: implications for structure rearrangements in the helicase hexamer., Weigelt J, Brown SE, Miles CS, Dixon NE, Otting G, Structure. 1999 Jun 15;7(6):681-90. PMID:10404597 Page seeded by OCA on Fri May 2 22:00:27 2008