1jvo: Difference between revisions

Revision as of 21:58, 2 May 2008

Azurin dimer, crosslinked via disulfide bridge

OverviewOverview

The transfer of electrons between proteins is an essential step in biological energy production. Two protein redox partners are often artificially crosslinked to investigate the poorly understood mechanism by which they interact. To better understand the effect of crosslinking on electron transfer rates, we have constructed dimers of azurin by crosslinking the monomers. The measured electron exchange rates, combined with crystal structures of the dimers, demonstrate that the length of the linker can have a dramatic effect on the structure of the dimer and the electron transfer rate. The presence of ordered water molecules in the protein-protein interface may considerably influence the electronic coupling between redox centers.

About this StructureAbout this Structure

1JVO is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.

ReferenceReference

Dramatic modulation of electron transfer in protein complexes by crosslinking., van Amsterdam IM, Ubbink M, Einsle O, Messerschmidt A, Merli A, Cavazzini D, Rossi GL, Canters GW, Nat Struct Biol. 2002 Jan;9(1):48-52. PMID:11740504 Page seeded by OCA on Fri May 2 21:58:57 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1jvo.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1jvo |SIZE=350|CAPTION= <scene name='initialview01'>1jvo</scene>, resolution 2.75&Aring;
+The line below this paragraph, containing "STRUCTURE_1jvo", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE= azu ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa])
+-->
-|DOMAIN=
+{{STRUCTURE_1jvo|  PDB=1jvo  |  SCENE=  }}
-|RELATEDENTRY=[[1jvl|1JVL]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jvo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jvo OCA], [http://www.ebi.ac.uk/pdbsum/1jvo PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1jvo RCSB]</span>
-}}
 '''Azurin dimer, crosslinked via disulfide bridge'''
@@ Line 33: / Line 30: @@
 [[Category: Rossi, G L.]]
 [[Category: Ubbink, M.]]
-[[Category: covalent crosslink]]
+[[Category: Covalent crosslink]]
-[[Category: cupredoxin]]
+[[Category: Cupredoxin]]
-[[Category: electron transfer]]
+[[Category: Electron transfer]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 21:58:57 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:39:39 2008''